FastQCFastQC Report
Wed 4 Jan 2023
EGAF00002439753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13613122
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC363260.2668454745355253Illumina Single End PCR Primer 1 (97% over 35bp)
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC257610.18923653222236603No Hit
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGA249710.18343330795096083Illumina Single End PCR Primer 1 (97% over 35bp)
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC221790.16292368495632376No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT170350.12513661451061703No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG149920.11012903579355271No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT140680.10334146715206106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)