FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4896920
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG924991.8889220162877889TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA886901.811138429870204TruSeq Adapter, Index 7 (97% over 38bp)
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT359310.733746926639602No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC238140.48630567785465145No Hit
CGTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT216760.4426455813041667TruSeq Adapter, Index 7 (97% over 38bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT215810.44070558636857454No Hit
CGTATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA215500.4400725353895918TruSeq Adapter, Index 7 (97% over 38bp)
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT214200.4374178054777289No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGT194590.3973722258072421TruSeq Adapter, Index 7 (96% over 33bp)
CGTATGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC194170.39651454383571716TruSeq Adapter, Index 7 (97% over 38bp)
CGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCT186170.3801777443780989TruSeq Adapter, Index 7 (97% over 38bp)
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC178540.3645965218953955No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG171100.3494032983998105No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTAGAT168650.3444001535659149TruSeq Adapter, Index 7 (97% over 38bp)
CGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATC160460.32767535512117824TruSeq Adapter, Index 7 (97% over 38bp)
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT157790.3222229483021981No Hit
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC134070.27378433791036TruSeq Adapter, Index 7 (97% over 38bp)
CGTATGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC126750.25883616640663926TruSeq Adapter, Index 7 (97% over 38bp)
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT125810.25691659247036913No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC124240.2537104955768115No Hit
CGTATGCCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT118950.24290778693546147TruSeq Adapter, Index 7 (97% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGT114830.23449433521478807TruSeq Adapter, Index 7 (97% over 38bp)
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT112770.2302876093544514No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA103160.21066302900598746No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG98190.2005137923429421No Hit
CCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT86670.1769888011239718TruSeq Adapter, Index 7 (97% over 38bp)
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC85590.17478333319719333No Hit
CGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA81900.1672479844473669TruSeq Adapter, Index 7 (97% over 37bp)
CGTATGCCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA81560.16655367047041814TruSeq Adapter, Index 7 (97% over 37bp)
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA80050.1634700995727927No Hit
CGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT78580.16046821267245534TruSeq Adapter, Index 7 (97% over 38bp)
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT75900.1549953848541532No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG75390.15395391388873006No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG67810.13847479640263677No Hit
GGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT64080.13085776365552224TruSeq Adapter, Index 7 (97% over 38bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA63230.12912197871315031TruSeq Adapter, Index 7 (97% over 37bp)
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT63100.128856505721964No Hit
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC62840.1283255597395914TruSeq Adapter, Index 7 (97% over 38bp)
CGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCT62780.12820303374365927TruSeq Adapter, Index 7 (97% over 38bp)
GACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCC59710.12193378695179828TruSeq Adapter, Index 7 (97% over 38bp)
ACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA56410.11519485717553074TruSeq Adapter, Index 7 (97% over 38bp)
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG53740.10974245035655064No Hit
CGTATGCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT53160.10855803239587332TruSeq Adapter, Index 7 (97% over 38bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA51680.10553572449621394TruSeq Adapter, Index 7 (97% over 38bp)
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA49920.10194162861553793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)