FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002439755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4896920
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC890911.8193272505983353Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGA837761.7107896392017838Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC345270.705075843591482No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC336970.6881264141542031No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT214740.43852053944111813No Hit
GCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGT211010.4309035066940036Illumina Single End PCR Primer 1 (96% over 33bp)
CATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTG203900.4163841761760454Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT202530.4135864992689282No Hit
GGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCG188280.3844865752350457Illumina Single End PCR Primer 1 (96% over 33bp)
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG184450.37666533249471096No Hit
TACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTA179520.36659777982895375Illumina Single End PCR Primer 1 (96% over 33bp)
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT166710.34043847969744245No Hit
ATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGT155670.31789369644592924Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTCGGTG155350.3172402244676245Illumina Single End PCR Primer 1 (96% over 27bp)
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT153260.3129722356093218No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC136740.27923674472934007No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT129860.26518709719578837No Hit
ACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAG128400.26220563129477303Illumina Single End PCR Primer 1 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCC121690.2485031407496957Illumina Single End PCR Primer 1 (96% over 33bp)
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT119390.24380631090563046No Hit
GACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG114160.23312612826021256Illumina Single End PCR Primer 1 (96% over 33bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAT113170.23110444932733226Illumina Single End PCR Primer 1 (96% over 33bp)
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT111270.22722445945614794No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC107510.2195461637110674No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG106470.217422379781577No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC101230.20672177613683704No Hit
CGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTG89790.18336015291244293Illumina Single End PCR Primer 1 (96% over 33bp)
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG89010.18176731496532514No Hit
CGGTGGTCGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGA85300.17419112421685468Illumina Single End PCR Primer 1 (96% over 33bp)
CCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTG79790.1629391535904201Illumina Single End PCR Primer 1 (96% over 33bp)
AGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGA78420.16014147668330297Illumina Single End PCR Primer 1 (96% over 33bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAG64420.131552077632471Illumina Single End PCR Primer 1 (96% over 33bp)
ACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTA64180.13106197364874247Illumina Single End PCR Primer 1 (96% over 33bp)
CGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGA62680.12799882375043903Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG60520.12358788789688213Illumina Single End PCR Primer 1 (96% over 33bp)
AAGTGCAATTTCCGTTCTAAGTGCAATTTCCGTTCTAAGTGCAATTTCCG60440.12342451990230595No Hit
CGGTGGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCG60310.12315904691111963Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGT58010.11846221706705438Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA57810.11805379708061395No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT57620.1176657980934955No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT57240.11688980011925865No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG56540.11546033016671703No Hit
ACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC51570.1053110935036717Illumina Single End PCR Primer 1 (96% over 33bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGT51420.10500477851384137Illumina Single End PCR Primer 1 (96% over 33bp)
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG49560.1012064726399451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)