FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002439758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14633784
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT3652222.4957454613242893No Hit
CCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAAT2839041.940058702520141TruSeq Adapter, Index 13 (97% over 38bp)
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC2165861.4800409791479772No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT2124891.45204411927906No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC1433090.9793024141944421No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG1361940.9306820436874017No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT1321320.9029243564070647No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG1007510.6884822134862726No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT964900.6593646592022953No Hit
ACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAA844420.5770346207105421TruSeq Adapter, Index 13 (97% over 38bp)
CACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATC774740.5294187750755375TruSeq Adapter, Index 13 (97% over 38bp)
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC767690.5246011557912841No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT694920.474873757874245No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA686770.46930445331159737No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA623230.42588437823053826No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT584170.39919271734501477No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA504510.34475703618421594TruSeq Adapter, Index 13 (97% over 38bp)
GACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAA482640.32981216614923387TruSeq Adapter, Index 13 (97% over 38bp)
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA386130.26386203322394264No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG352220.24068962614180994No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT351480.24018394695452658No Hit
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCG350260.23935025964576218TruSeq Adapter, Index 13 (97% over 38bp)
CCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTC344380.23533216015761885TruSeq Adapter, Index 13 (97% over 38bp)
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC342970.23436863630076815No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG335760.22944168097602094No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG331880.22679028199404883No Hit
CGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA319450.21829623834819487TruSeq Adapter, Index 13 (97% over 38bp)
ACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCT285910.19537667085970384TruSeq Adapter, Index 13 (97% over 38bp)
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT272080.18592593685952996No Hit
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT264960.1810604830575605No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA258920.17693304752892347No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC249160.17026354905880803No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG242800.1659174414491836No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA239670.1637785551570257No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA238690.1631088719090018No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC230090.15723205973246565No Hit
TACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGG170890.11677772474979814No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG169840.11606020698405825No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA159440.10895336435196802No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC157730.10778483541919165No Hit
TGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA151320.10340456029691295Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC148290.10133400903006358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)