FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002439759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14633784
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC4084202.7909391036522067No Hit
CGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTG2974242.032447656737314Illumina Single End PCR Primer 1 (96% over 33bp)
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC2506201.71261240428313No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT1541001.0530427400049092No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT1502481.0267200882560519No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT1379160.9424493350455356No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG1247000.8521377655977429No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT1016970.6949467068804623No Hit
CGGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGT867670.5929225140947824Illumina Single End PCR Primer 1 (96% over 33bp)
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT852720.5827064278111526No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT849630.5805948755291181No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC818090.5590420085467983No Hit
CGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGT816070.5576616410355654Illumina Single End PCR Primer 1 (96% over 33bp)
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT742340.507278226875564No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC700120.47842717919029004No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG640880.43794551019749917No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC631760.4317133558893585No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC518400.35424877119957493Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG488280.33366626157663665Illumina Single End PCR Primer 1 (96% over 33bp)
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG371880.25412429211747284No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG370350.25307876623025183No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG368360.2517188992266115No Hit
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAG354410.2421861631960674Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTAT324830.22197266270979535Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGA323640.2211594759086235Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA311270.21270643327795463No Hit
CGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTA297250.20312586272969452Illumina Single End PCR Primer 1 (96% over 33bp)
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT293270.2004061287224138No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA289870.19808273786192282No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG262970.17970061605392015No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC261790.17889426275527917No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA256720.17542967697213516No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA241070.16473524551134552No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT231260.1580315795285758No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA175920.1202149765228187No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC167830.11468667297535622No Hit
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC164570.11245895115029715No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG151530.10354806385006092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)