FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002439767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20199812
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTG1095940.5425496039270068Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGA481500.2383685551132852Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGT390900.19351665253122158Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC310810.15386776867032229Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGT310300.15361529107300603Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATG236070.1168674243106817Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)