FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002439813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6376379
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT389560.6109423545871411No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC326110.5114344677441538No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC317930.49860587019686253No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT308840.4843501303796402No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC249460.39122517654612443Illumina Single End PCR Primer 1 (97% over 35bp)
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT210360.32990510758535524No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT203340.3188957243601737No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT184710.28967851503180725No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC178430.279829665081075No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG149200.23398860074032612No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT146360.22953466222757463No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN144110.22600601375796517No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC130980.20541438957753294No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG124410.1951107360462733No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG123960.1944050063523514No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT114760.17997675483217043No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC94020.14745045738341464No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT93270.14627424122687813No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG86130.13507666341665073No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT78020.12235784604396947No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG77160.1210091181844743No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA67970.10659654954638048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)