FastQCFastQC Report
Fri 31 Mar 2023
EGAF00002439814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13682256
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT641220.468650783905812No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC611450.44689267617854833No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT537050.39251567870093934No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA355490.25981826388864526TruSeq Adapter, Index 27 (97% over 39bp)
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG347320.25384702639681644No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT330730.24172183300765604No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT304310.22241215191412875No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG279170.20403798905677542No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC265860.19431006114781071No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT256260.18729367437650635No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT246020.17980952848711498No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC226690.16568174137364483No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT213470.15601959209066107No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG205770.15039186520117734No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT201930.1475853104926556No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA196960.14395286859126155No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC162460.11873772863188643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)