FastQCFastQC Report
Sat 1 Apr 2023
EGAF00002439815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13682256
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT648980.47432236321261645No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC566590.41410568549514054No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC565580.41336750313690956No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT538890.3938604861654394No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC366080.2675582155457404Illumina Single End PCR Primer 1 (97% over 35bp)
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT352410.25756717313285177No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT346100.2529553605779632No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC324110.23688344962994407No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT319710.23366760569309622No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT255510.18674551915999818No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG250160.18283534528224No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC226950.16587176851536764No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG210460.15381966248840834No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG208380.15229944535462572No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT195220.14268114848896263No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC159520.11658896018317447No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT159400.11650125534853316No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG149820.109499486049669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)