FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6302729
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA751821.1928483677467332TruSeq Adapter, Index 6 (97% over 36bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT595430.9447177563877489No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC450290.714436555974404No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT393370.6241264696609993No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG274970.4362713357975569No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT251400.3988748365985591No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT237100.3761862520187684No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT236950.3759482598728265No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG225250.35738487248936135No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT220110.3492296749550869No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC211050.33485494934019855No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT209600.33255435859609384No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG201280.31935372756785196No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC191770.30426502551513795No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT188900.299711442456117No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC175700.27876813361323327No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA161180.2557304938860611No Hit
CAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGAC127360.20207119804770282No Hit
CGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAG127040.20156348146969355No Hit
GGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGT115210.18279383422641207No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT111980.17766907001713067No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG105700.1677051321736981No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA103130.16362753340656086No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG101530.16108895051651437No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA98130.1556944618751655No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA85680.13594111376199103No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA83250.13208564099773287No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC81690.12961052267993753No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA79710.12646902635350496No Hit
CGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGCTA76050.12066201799252356No Hit
GCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATG74410.11805997053022588No Hit
TACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCG74170.11767918309671889No Hit
ACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGA72890.11564831678468168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)