FastQCFastQC Report
Fri 31 Mar 2023
EGAF00002439826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14588510
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT1111550.761935249041883No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA1020950.6998315797843645TruSeq Adapter, Index 6 (97% over 36bp)
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC934360.6404766490889063No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT772600.5295948660966747No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG515240.3531820590313884No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT472670.32400156013191206No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT470740.32267860117311503No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT450400.3087361217835132No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG434190.2976246374715444No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC404440.2772318763191032No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT388950.26661393110057163No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT387610.26569540001000785No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG365990.25087551778762873No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC360650.24721510284463596No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT343470.2354387116984531No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC315310.21613584937735247No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA298540.2046405013260436No Hit
CGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAG227200.15573900281797112No Hit
CAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGAC225470.15455313805179557No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT202660.1389175453833188No Hit
GGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGT197760.13555873766409318No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA196900.13496923263582095No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG194910.13360514541923746No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG182080.12481055296257122No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA176580.12104046266548127No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA157880.10822215565537535No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA154740.10606977683121853No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA145900.10001021351735029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)