FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002439836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6750941
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA353660.5238677097015068TruSeq Adapter, Index 7 (97% over 38bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT148400.2198212071472703No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC129320.19155848051404983No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT115210.1706576905352898No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT73680.10914034058363124No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG71520.10594078662515344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)