FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002439838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14119851
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA465550.3297131109952931TruSeq Adapter, Index 7 (97% over 38bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT234270.16591534854015103No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC221640.1569704949436081No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT202230.14322389096032245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)