FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14119851
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC488410.3459030835382045Illumina Single End PCR Primer 1 (97% over 35bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT240470.17030632971976828No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC203910.14441370521544455No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC194890.13802553582187235No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT191310.13549009830202882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)