FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002439865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5627983
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT1205832.142561553579675No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC1002911.782006093479671No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT898941.5972685063192267No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC823721.4636149398461225No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT675641.2005011386850315No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT563881.0019220029626956No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC505750.8986345552216488No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT449390.7984921063194398No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT415500.7382751511509541No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG412890.7336376104902947No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC406160.7216795075607016Illumina Single End PCR Primer 1 (96% over 33bp)
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC400480.7115870819083853No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG377410.6705954868733612No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG355670.6319670830562211No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT297130.5279511327592852No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC278640.49509744432419217No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT272020.48333479329983764No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT254200.45167158465119744No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT226110.40176027539528814No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG224410.3987396550416019No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG213200.3788213290622946No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT207890.3693863325457806No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA194930.34635854443767866No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA187260.3327302161360473No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC162970.28957088178837787No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA155980.277150801628221No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC155630.27652890920246204No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG134630.23921536365692644No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC133310.23686994079406423No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG128990.22919401142469692No Hit
GGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGT125420.22285070868195586No Hit
CGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAG121190.21533469450778367No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA116460.20693026258252734No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN116050.20620176002663831No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA109440.19445687735730546No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA93880.16680931694356574No Hit
GCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATG93390.16593866754750325No Hit
CAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGAC86970.15453138362358237No Hit
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC80610.14323070982979158No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG79090.14052991986649568No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCAGATCGG77910.13843325397393702No Hit
GACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGAGATCGG75940.13493288803466535No Hit
ACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGA67700.12029176349679804No Hit
ACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGA66280.11776865708371899No Hit
TGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAA64190.11405507088418709No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCAGA63260.11240261386717054No Hit
TACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCG62870.11170964802132487No Hit
ATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTA61580.1094175302235277No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA61490.10925761502833253No Hit
ACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATC60740.10792498840170626No Hit
TTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGA60190.10694772887551367No Hit
ACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGAGATCGGA58560.10405148700697922No Hit
TCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGT57600.1023457249248976No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTAGATCGGA57460.10209696795459404No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)