FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12315125
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT2175911.7668598572893088No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC1792121.4552186843414094No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT1407861.1431958668710223No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG1027520.8343561271201064No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT1013690.8231260340435035No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT974260.7911084946356615No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC804880.6535703048081121No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT801210.6505902294942195No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT791450.6426650155804345No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT791070.6423564519239553No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT755080.6131322256168735No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG753720.6120278925305265No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC748630.6078947635529481No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG716640.5819185757351224No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC633430.5143512550623724No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA566140.45971112757686183No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA556600.451964555779986TruSeq Adapter, Index 19 (97% over 38bp)
CGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAG478170.3882786411018971No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG456860.37097471605038523No Hit
GGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGT432020.3508043970321048No Hit
CAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGAC405660.32939982338790713No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG383350.3112838887140813No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT363560.2952142182884867No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA353750.28724840389358614No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA342830.278381258817917No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA339910.27601019072076005No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA332460.26996071903452057No Hit
GCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATG299920.24353792592442222No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA283610.23029404898448047No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC271510.22046873255448077No Hit
ACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGA263830.21423249865510904No Hit
TACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCG253360.20573075790948125No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG250290.20323788836897716No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC250080.20306736634829126No Hit
ACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGA237510.19286040539580393No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG232060.18843495295419252No Hit
ATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTA223890.18180083433988692No Hit
CGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGCTA215650.17510987505201936No Hit
ACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATC212940.17290932897554837No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT206920.16802103104921795No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA190590.15476091391682992No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC172340.13994173830959897No Hit
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT164620.13367302402533468No Hit
AAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACG163430.13270673257478102No Hit
ACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGCT151420.12295449701078959No Hit
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC147470.11974705900264918No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC133920.10874432862029415No Hit
GACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGAGATCGG128620.10444067762202983No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCAGATCGG127860.10382355030907117No Hit
TGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAA125850.10219141096821999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)