FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16438357
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC1468770.8935017045803301No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT1382110.8407835405935032No Hit
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT1058900.6441641339216565No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT836320.5087613074713002No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG730140.4441684774214357No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG723170.43992839430363995No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC554340.3372234828578063No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT503540.3063201510953923No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA431870.2627209033116874No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT418070.2543259037384332No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC397060.24154482105480493No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG372450.22657373848250165No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT340660.20723482279889652No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA335240.2039376562998358No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT260870.15869590859962465No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT251600.1530566588862865No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA241150.1466995758761049TruSeq Adapter, Index 7 (97% over 36bp)
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC192660.11720149404225738No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA185560.11288232759514834No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG181810.11060107771111188No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG178990.10888557779831645No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC171960.1046089946823761No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT167610.10196274481689381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)