FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002439884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8071319
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA387350.4799091697404104TruSeq Adapter, Index 8 (97% over 36bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT224070.27761261821023303No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT193990.24034485565494315No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC192170.23808995778756853No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG126940.15727293147501667No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT118690.1470515537795No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT105150.13027610481013077No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC100100.12401938270560238No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT98790.12239635182304157No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT95030.11773788150363032No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG93970.11642458933911547No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC91840.11378561546136387No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT81590.1010863280214795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)