FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6755579
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA1560632.3101350750246574TruSeq Adapter, Index 1 (97% over 36bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT1006981.4905902218003815No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC783191.1593232793221722No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT740111.095553763785458No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG573340.848691133654125No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT518010.766788457362426No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT513070.7594759827395994No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT487170.7211373000004885No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT470680.6967278452372476No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC451300.668040444793851No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC450260.6665009764522034No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT428520.6343201670796833No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT416350.6163054269663636No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC380770.5636378465857627No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG351360.5201034581935907No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG342950.5076544882385359No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG282320.41790644443651687No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA271850.40240814295858285No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG254530.37677007403806545No Hit
CGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAG234530.3471649136217636No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT225350.333576144990681No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA214140.31698245257734387No Hit
GGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGT202630.29994468275776215No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA201360.29806475507132696No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA186310.27578687185805983No Hit
CAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGAC183390.27146451843727976No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC178340.2639892154321635No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA178250.26385599221029016No Hit
GCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATG167920.2485649268552703No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA161890.23963897098975528No Hit
ACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGA159360.2358939181970931No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC144890.21447458463589872No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG143830.21290551113383474No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG142910.21154367375468483No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT134210.19866542897359354No Hit
ACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATC127740.1890881595789199No Hit
ACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGA126190.18679375964665648No Hit
TACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCG125760.186157248697706No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA125370.1855799480695881No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC118210.17498130064055203No Hit
ATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTA118090.17480366967805425No Hit
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT110990.1642938377302671No Hit
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC94190.13942550298057355No Hit
CGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGCTA93490.13838932236600296No Hit
ACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGCT83900.12419364794638624No Hit
TGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAA82550.12219529961828585No Hit
AAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACG81550.12071504159747078No Hit
TACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGG81120.12007853064852028No Hit
AGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGACGCTAGC79820.11815419522146066No Hit
TCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGT79560.11776932813604875No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC78460.11614104431315214No Hit
TTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGA77910.11532690240170383No Hit
TCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATCCGC74310.10999797352676952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)