FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002439893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002439893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6755579
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC1588812.351848746051227Illumina Single End PCR Primer 1 (96% over 33bp)
CGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTT1059061.5676820595244316No Hit
CTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTC877771.2993260829308635No Hit
GCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCT877761.2993112803506555No Hit
GGCTAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCC856201.267396917421882No Hit
AGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGT600090.8882880357109287No Hit
GCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTT585170.8662025860403676No Hit
CGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGAC516960.7652341864405701No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTT495740.7338231112388739No Hit
TCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGT493400.7303593074701664No Hit
AACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAAC440440.6519648426877992No Hit
TAGCGTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCG432660.6404484352858577No Hit
GATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAG403120.59672161335098No Hit
GTCGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTG344610.5101117165530888No Hit
TCGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTT331750.4910755984054068No Hit
CACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGAT319360.47273520152750786No Hit
ATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGC296010.4381711767414755No Hit
ATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTG278310.41197060977304834No Hit
CATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGT271470.4018456449106731No Hit
CGCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTG236070.34944451097381884No Hit
CGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGAT216590.3206090847283408No Hit
GTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTT216540.32053507182730007No Hit
GGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACA192040.2842687503173303No Hit
CTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATC186570.27617173894347175No Hit
CGAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTA174150.2577869343249483No Hit
TTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTC170700.2526800441531362No Hit
GAACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAA161980.23977219421162863No Hit
CTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCG144700.21419333561194387No Hit
CTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCG143420.21229860534530054No Hit
ACTTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACC143420.21229860534530054No Hit
GCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGA139540.20655520422453796No Hit
GCGGATTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGA136470.20201081210063565No Hit
CGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAG117190.17347143745932064No Hit
GGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGT111960.16572968801045773No Hit
TCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAA107830.15961622238449139No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN106650.1578695179199296No Hit
GCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATG100360.14855869496900265No Hit
CATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGC94080.13926267459828387No Hit
AGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATG89670.1327347367264893No Hit
ACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATCGGA88820.13147651740879648No Hit
TTAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCA81520.12067063385684633No Hit
TGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAA80700.11945682227977794No Hit
TCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGT77830.11520848176003863No Hit
TCAAAGTGCATCAGGTCCTCGTTTGTAACCGATGCGTAGTGCTAATCCGC74280.10995356578614504No Hit
TTACATCCGATCATGCTGTCAAAGTGCATCAGGTCCTCGTTTGTAACCGA73860.10933185741740271No Hit
TAGCACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCAT72000.10657857749868664No Hit
CAAACGAGGACCTGATGCACTTTGACAGCATGATCGGATGTAAGTTCGAC70610.10452101884975365No Hit
ACTACGCATCGGTTACAAACGAGGACCTGATGCACTTTGACAGCATGATC69380.10270030148415109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)