FastQCFastQC Report
Wed 1 May 2024
EGAF00002440957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002440957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32873235
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTA27363168.323841568984616Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG8358642.542688603661915Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA1953450.5942372267286745Illumina Paired End Adapter 2 (100% over 32bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT602430.1832585080233205No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC522040.1588039631633455Illumina Paired End Adapter 2 (96% over 28bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGAGCTCGTA406340.12360815721361162Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)