FastQCFastQC Report
Wed 1 May 2024
EGAF00002440959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002440959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34624914
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGCTATCTCGTA352463010.179462106389636Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG12747693.681652465620564Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA2676730.7730647359875031Illumina Paired End Adapter 2 (100% over 32bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT842750.24339410633626413No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC788270.22765977122715741Illumina Paired End Adapter 2 (96% over 28bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGCTAGCTCGTA387910.1120320472131714Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)