FastQCFastQC Report
Wed 1 May 2024
EGAF00002440961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002440961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38914473
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15870234.0782333092369No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAACAATCTCGTA10554342.712188855801799Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG6182711.5887944827108413Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA2215870.5694205341030829Illumina Paired End Adapter 2 (100% over 32bp)
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA777420.19977657155989237No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC720840.18523699395852025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)