FastQCFastQC Report
Wed 1 May 2024
EGAF00002440963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002440963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39240028
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19942125.082086078022167No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTAGCTATCTCGTA15681783.9963732951464763Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG9087852.315964198598431Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA3597920.9169004670434996Illumina Paired End Adapter 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC553900.14115688194717904Illumina Paired End Adapter 2 (96% over 28bp)
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA540560.13775729212017893No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC526480.13416911934925224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)