FastQCFastQC Report
Thu 2 May 2024
EGAF00002440964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002440964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39240028
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG18443544.700185229225626Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT4638251.182020053604447Illumina Paired End Adapter 2 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2052370.5230296981439463No Hit
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT874410.22283623243082293Illumina Paired End Adapter 2 (96% over 30bp)
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA442920.11287453719451984Illumina Paired End Adapter 2 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)