FastQCFastQC Report
Thu 2 May 2024
EGAF00002440965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002440965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47101530
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40472078.592517058363072No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATCTCGTA19425964.124273670090972Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG13806492.9312190071108093Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA4979451.0571737266284131Illumina Paired End Adapter 2 (100% over 32bp)
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC2313020.49107109684122785No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA2154450.45740552376961No Hit
GATCGGAAGAGCGGTTCAGCAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1438830.30547415338737405Illumina Paired End PCR Primer 2 (100% over 27bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC804680.1708394610536006Illumina Paired End Adapter 2 (96% over 28bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGATCGGAAGAGCGGTTCAGCAGGA492480.10455711311288614Illumina Paired End Adapter 2 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)