FastQCFastQC Report
Sun 12 May 2024
EGAF00002440966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002440966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47101530
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG23512484.9918718139304605Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT6852541.454844460466571Illumina Paired End Adapter 2 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4991721.0597787375484404No Hit
CCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAA1180310.2505884628376191Illumina Single End PCR Primer 1 (100% over 27bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT1153970.24499628780636212Illumina Paired End Adapter 2 (96% over 30bp)
GCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTT843800.17914492374239224No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC806630.17125346034406952No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG652730.13857936249629257No Hit
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA595810.12649482936116938Illumina Paired End Adapter 2 (96% over 29bp)
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG511910.10868224450458404No Hit
GCTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG501050.10637658691766488Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)