FastQCFastQC Report
Wed 27 Apr 2022
EGAF00002441058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15547227
Sequences flagged as poor quality0
Sequence length0-34
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGTAATTCAGGATAGGTT12285267.901897875421772No Hit
TAGCAGCACGTAAATATTGGCG7418574.77163548200589No Hit
TAGCTTATCAGACTGATGTTGAC3573982.2987893596716638No Hit
TTCAAGTAATCCAGGATAGGCT3415762.197022015565863No Hit
GGTTTCGTAAACCGAAGATC3381082.174715786937439No Hit
CACAGATGATGAACTTATTG1868611.2018927876977676No Hit
TCCCTACACGACGCTCTCCCTACACG1865441.1998538388871534No Hit
AGGGGTAAAGCACTGTTACGTAT1364130.8774104861272046No Hit
ACCTCAGAAGGTCTCACTTTT1275420.8203520795058823No Hit
TAGCTTATCAGACTGATGTTGA1267130.8150199389254431No Hit
ACAGATGATGAACTTATTG1121970.721652806638766No Hit
TAGCTTATCAGACTGATGTTG943380.606783447620595No Hit
ATCACATTGCCAGGGATTACC942470.60619813423963No Hit
TAGCAGCACGTAAATATTGGCGT932740.5999397834739275No Hit
TCCCTACACGACGCTCTCCCTAC837680.5387970472162014No Hit
AACCGGGCGGAAACACCA755270.48579081015540587No Hit
CAAAGTGCTTACAGTGCAGGTAG626380.4028885665591684No Hit
AAAGGTTCGTTTGTAAAAAA601270.38673777645364027No Hit
ACTGGACTTGGAGTCAGAAGGC590120.3795660795330254No Hit
AAAGGTTCGTTTGTAAAAA588930.37880066972714815No Hit
TATGGCTTTTCATTCCTATGTGA573320.36876029403828736No Hit
TGAGGTAGTAGATTGTATAGTT571000.3672680665175854No Hit
GGTATCGTAAACCGAAGATC535700.3445630529482846No Hit
CAAAGTGCTTACAGTGCAGGTAGT525860.3382339500156523No Hit
AAAGGTTCGTTTGTAAAAAAA522570.3361178170229328No Hit
TTCAAGTAATCCAGGATAGGCTT465340.2993073941738935No Hit
TGAGGTAGTAGTTTGTACAGTT455950.293267731924156No Hit
TAAAGGTTCGTTTGTAAAAA430360.27680820509020676No Hit
CAGTGGTTTTACCCTATGGTAG426460.2742997191717854No Hit
TTCAAGTAATTCAGGATAGGTTT423230.2722221782701185No Hit
TAAAGTGCTTATAGTGCAGGTAG422200.2715596807070483No Hit
TAGGGGTAAAGCACTGTTACGTAT412860.26555217853318797No Hit
AAAGGTTCGTTTGTAAAAAAAA398500.2563158047412571No Hit
TGTAACAGCAACTCCATGTGGA397150.2554474826925728No Hit
TTCAAGTAATCCAGGATAGGC379110.2438441273160802No Hit
CAACGGAATCCCAAAAGCAGCT373540.24026149486336051No Hit
AATGACACGATCACTCCCGTTGAGT371160.23873067525160596No Hit
TAAAGGTTCGTTTGTAAAAAA368030.23671745450169346No Hit
TGTAACAGCAACTCCATGTGG367910.23664027031958818No Hit
TAGGATGGGGTGTGATAGGTGGC367260.23622218933318462No Hit
ATCACATTGCCAGGGATTTCC353140.2271401839054643No Hit
CTGACCTATGAATTGACAGCC327250.21048769661625188No Hit
TAAAGGTTCGTTTGTAAAAAAA311350.20026079248730336No Hit
AGCTCGGTCTGAGGCCCCTCAGT307590.1978423547813382No Hit
AAGGTTCGTTTGTAAAAAAA306800.19733422558247848No Hit
CAACGGAATCCCAAAAGCAGCTG305670.19660740786765382No Hit
GGTGTCGTAAACCGAAGATC291440.18745465027300365No Hit
AAGGTTCGTTTGTAAAAAA288410.18550574967484557No Hit
TGTTTCGTAAACCGAAGATC286860.18450878732265247No Hit
ACCTCAGAAGGTCTCACTTT278300.17900298233247638No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC273440.1758770229572129No Hit
TGGCAGTGTCTTAGCTGGTTGTT273220.1757355186233532No Hit
AAAGGTTCGTTTGTAAAAAAAAA271950.17491865269607243No Hit
AGTAAGGTCAGCTAAATAAGCTA261480.16818433280738745No Hit
TCCCTAC254230.16352112180519396No Hit
TGAGGTAGTAGGTTGTATAGTT252830.16262063968063245No Hit
AAGGTTCGTTTGTAAAAAAAA250620.16119916432686035No Hit
CAAAGTGCTGTTCGTGCAGGTAG249700.16060741893071995No Hit
TAAAGGTTCGTTTGTAAAAAAAA246480.15853631004422847No Hit
TGGCTCAGTTCAGCAGGAACA243320.15650379324878963No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT243280.15647806518808788No Hit
TTCGTGATCGATGTGGTGACG243190.15642017705150893No Hit
TAAAGGTTCGTTTGTAAAA236010.1518019901555435No Hit
TAAAGTGCTGACAGTGCAGATA231090.14863743868922732No Hit
TCGAGGAGCTCACAGTCTAGTA230930.14853452644642032No Hit
AAGGAGCTCACAGTCTATTGAG228610.14704229892571838No Hit
AGCAGCATTGTACAGGGCTATGA224310.1442765324002795No Hit
TAAAGTGCTTATAGTGCAGGTA224080.14412859605124437No Hit
TAGGATGGGTGTGATAGGTGGC222110.14286148906168283No Hit
TTCACAGTGGCTAAGTTCTGC221770.1426428005457179No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA220540.14185166267913887No Hit
TGAGAACTGAATTCCATGGGTT218070.14026295493080534No Hit
TTCAAGTAATTCAGGATAGGT216960.13954900124633157No Hit
TTTGGCAATGGTAGAACTCACACT216190.13905373607782273No Hit
AAGCTGCCAGTTGAAGAACTGT211230.13586345655080484No Hit
TGAGGTAGTAGTTTGTGCTGTT208750.1342683167872959No Hit
TAAAGTGCTGACAGTGCAGAT204270.13138677398869908No Hit
AAAGGTTCGTTTGTAAAAAAAAAA204190.13133531786729558No Hit
ATATAATACAACCTGCTAAGTG200440.12892331217650582No Hit
AAAGGTTCGTTTGTAAAA198730.1278234375815057No Hit
TAGCAGCACGTAAATATTGGC196040.1260932254993125No Hit
TCGAGGAGCTCACAGTCTAGT194840.12532138367825982No Hit
CAAAGTGCTTACAGTGCAGGTA194500.12510269516229486No Hit
GAATCCCGAGTGTTGTTCTC192740.12397066049141753No Hit
CTTCGTGATCGATGTGGTGACG190210.12234336065203139No Hit
TAAGGTGCATCTAGTGCAGATAG185960.11960975420246968No Hit
AAGGTTCGTTTGTAAAAA185070.11903730485185557No Hit
TGTAACAGCAACTCCATGTGGAA184090.11840696736466252No Hit
TAGCTTATCAGACTGATGTTGACA183090.11776376584711858No Hit
AATCCCGAGTGTTGTTCTC181380.11666389125211847No Hit
TGGCTCAGTTCAGCAGGAAC180070.11582129726413593No Hit
TGTAAACATCCCCGACTGGAAGCT178800.11500443133685512No Hit
TAGCACCATCTGAAATCGGTT177690.11429047765238134No Hit
AAGGTTCGTTTGTAAAAAAAAA172870.1111902463378196No Hit
TAAAGGTTCGTTTGTAAAAAAAAA165710.10658492347220504No Hit
TGTAAACATCCTTGACTGGAAGCT165460.10642412309281907No Hit
TCAGTGCACTACAGAACTTTGT164640.10589669784843304No Hit
GCCCGGATAGCTCAGTCGGTAGAGC163770.10533711252816982No Hit
CAGTGCAATGTTAAAAGGGCATT162110.10426939800904687No Hit
TGTAAACATCCCCGACTGGAAGC160500.10323384356580115No Hit
GTTTCCGTAGTGTAGTGGTTATC156850.10088615802676579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)