FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002441059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11091372
Sequences flagged as poor quality0
Sequence length0-34
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG10693689.641440211364293No Hit
TTCAAGTAATTCAGGATAGGTT3273512.9514022250809004No Hit
GGTTTCGTAAACCGAAGATC2897472.6123639167453763No Hit
TCCCTACACGACGCTCTCCCTACACG2356712.1248137741660815No Hit
TAGCAGCACGTAAATATTGGCGT1991941.795936517141432No Hit
TCCCTACACGACGCTCTCCCTAC1834271.6537809749776673No Hit
TTCAAGTAATCCAGGATAGGCT1654831.4919975635115295No Hit
CACAGATGATGAACTTATTG1385121.2488265653699109No Hit
AACCCGTAGATCCGATCTTGTG1027050.9259900398255508No Hit
ACCTCAGAAGGTCTCACTTTT977440.8812615788200053No Hit
CAAAGTGCTTACAGTGCAGGTAG956260.8621656545285831No Hit
AACCCGTAGATCCGATCTTGT845250.7620788483156097No Hit
ATCACATTGCCAGGGATTACC775210.698930664303749No Hit
ACAGATGATGAACTTATTG764010.6888327251128175No Hit
TAGCTTATCAGACTGATGTTGAC762650.68760654678249No Hit
TAAAGTGCTTATAGTGCAGGTAG584960.5274009383149352No Hit
CAAAGTGCTTACAGTGCAGGTAGT576470.5197463397675237No Hit
AGGGGTAAAGCACTGTTACGTAT476970.43003696927665935No Hit
TGAGGTAGTAGGTTGTATAGTT447030.4030430139751872No Hit
GGTATCGTAAACCGAAGATC440540.3971916188547278No Hit
TAAAGTGCTTATAGTGCAGGTA407720.36760105061844467No Hit
AACCCGTAGATCCGATCTTGTGT392420.3538065444022615No Hit
AACCGGGCGGAAACACCA363430.32766911072859156No Hit
TAGCAGCACGTAAATATTGGC339100.3057331410397199No Hit
ACCATCGACCGTTGATTGTACC333960.3010989082324531No Hit
TGAGGTAGTAGATTGTATAGTT316570.28542005443510504No Hit
CAAAGTGCTTACAGTGCAGGTA316460.2853208782466227No Hit
AAAGGTTCGTTTGTAAAAAA309200.27877524980678675No Hit
ATCACATTGCCAGGGATTTCC299150.2697141525863527No Hit
ACACGACGCTCTTCCGATCTCAC288960.260526831126032No Hit
AACCCGTAGATCCGATCTTGTGA283220.2553516372906796No Hit
AAAGGTTCGTTTGTAAAAA283040.255189348982254No Hit
AAAGGTTCGTTTGTAAAAAAA277310.25002317116403633No Hit
GGTGTCGTAAACCGAAGATC274510.24749868636630346No Hit
CTGACCTATGAATTGACAGCC260680.23502953466893006No Hit
TCCCTAC255390.23026006160464188No Hit
CTGCAGTGAAGGCACTTGTAGC250430.22578811710580077No Hit
CAAAGTGCTTACAGTGCAGGT250160.22554468464316227No Hit
CTTCGTGATCGATGTGGTGACG245800.22161370117240678No Hit
TAAGGTGCATCTAGTGCAGATAG245730.2215505890524635No Hit
TAAAGTGCTTATAGTGCAGGT235290.21213786716377378No Hit
CCTACACGACGCTCTCCCTACACG219740.19811796051922162No Hit
TTCAAGTAATCCAGGATAGGCTT219170.19760404754254027No Hit
GCCGAAATAGCTCTCCCTACACG213850.19280752642684781No Hit
CACGACGCTCTTCCGATCTCC211550.19073384248585296No Hit
TAAAGGTTCGTTTGTAAAAA209950.18929127974429133No Hit
AAAGGTTCGTTTGTAAAAAAAA209630.189002767195979No Hit
TTTGGCAATGGTAGAACTCACACT207500.18708235554627506No Hit
AGTAAGGTCAGCTAAATAAGCTA205640.18540537635920967No Hit
TTCAAGTAATCCAGGATAGGC203200.18320546817832817No Hit
TTCGTGATCGATGTGGTGACG200830.18106867211738997No Hit
AACATTCAACGCTGTCGGTGAGT195830.17656066355000985No Hit
TAGCTTATCAGACTGATGTTGA195250.17603773455619376No Hit
TGAGGTAGTAGTTTGTACAGTT191020.1722239593081902No Hit
TAAAGGTTCGTTTGTAAAAAA186450.16810363947760476No Hit
TGAGAACTGAATTCCATGGGTT182380.16443412050375733No Hit
ACCATCGACCGTTGATTGTAC182140.1642177360925231No Hit
TGTAACAGCAACTCCATGTGG181810.163920207527076No Hit
AATCCCGAGTGTTGTTCTC181800.16391119150994124No Hit
TGGCTCAGTTCAGCAGGAACA180810.16301860581359998No Hit
GAATCCCGAGTGTTGTTCTC180190.16245961275124485No Hit
TAGCTTATCAGACTGATGTTG175520.1582491327493118No Hit
TAGGATGGGGTGTGATAGGTGGC170240.1534886757021584No Hit
TCGAGGAGCTCACAGTCTAGTA170070.15333540341086746No Hit
TGTAACAGCAACTCCATGTGGA164440.14825938576399747No Hit
AAGGTTCGTTTGTAAAAAAA161490.14559966070924318No Hit
AGCTCGGTCTGAGGCCCCTCAGT159770.14404890576206442No Hit
GCCCGGATAGCTCAGTCGG158400.14281371141460225No Hit
TAAAGGTTCGTTTGTAAAAAAA157370.14188506164972198No Hit
TTCACAGTGGCTAAGTTCTGC154060.13890075997811632No Hit
AAGCTGCCAGTTGAAGAACTGT151150.13627709899190107No Hit
AACCCGTAGATCCGAACTTGTG146990.13252643586384083No Hit
AAGGTTCGTTTGTAAAAAA146260.13186826661300333No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT143620.12948803808942663No Hit
TAGGGGTAAAGCACTGTTACGTAT142900.1288388848557239No Hit
TCGAGGAGCTCACAGTCTAGT142150.12816268357061686No Hit
AAGGAGCTCACAGTCTATTGAG141580.12764877059393553No Hit
GGCTGTAGGAATACTTTT137580.12404236374003144No Hit
GTTTCCGTAGTGTAGTGGTTATC135490.12215801615886655No Hit
AAGGTTCGTTTGTAAAAAAAA133720.12056218112601398No Hit
AAAGGTTCGTTTGTAAAAAAAAA130060.11726231885469175No Hit
TTTGGCACTAGCACATTTTTGCT129590.11683856604935801No Hit
ATCCCGAGTGTTGTTCTC127450.1149091383825193No Hit
AGCAGCATTGTACAGGGCTATGA127370.11483701024544123No Hit
TGTAAACATCCCCGACTGGAAGC127190.11467472193701556No Hit
ACCTCAGAAGGTCTCACTTT125200.11288053452719825No Hit
TAGCAGCACGTAAATATTGGCGA124060.11185270857383559No Hit
TAGTAGCACGTAAATATTGGCG116970.10546035242529057No Hit
CAAAGTGCTGTTCGTGCAGGTAG116950.10544232039102107No Hit
CAACGGAATCCCAAAAGCAGCT114980.1036661650154733No Hit
TTGGAATCCCGAGTGTTGTTCTC114360.10310717195311815No Hit
TAAAGGTTCGTTTGTAAAAAAAA113640.10245801871941543No Hit
AACATTCATTGCTGTCGGTGGGTT113180.10204328193121645No Hit
GCCCGGATAGCTCAGTCGGTAGAGC111720.10072694342954144No Hit
TAGGATGGGTGTGATAGGTGGC110970.10005074214443443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)