FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16811549
Sequences flagged as poor quality0
Sequence length0-34
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG405360224.112007763234665No Hit
TAGCAGCACGTAAATATTGGCGT5295713.1500428663652587No Hit
TTCAAGTAATTCAGGATAGGTT2657921.5810083889354871No Hit
GGTTTCGTAAACCGAAGATC2204981.3115864576191045No Hit
TTCAAGTAATCCAGGATAGGCT2202771.3102718851189739No Hit
TCCCTACACGACGCTCTCCCTACACG2091901.2443231733137736No Hit
TAGCTTATCAGACTGATGTTGAC2040021.2134634351659088No Hit
CACAGATGATGAACTTATTG1936561.151922407625853No Hit
TAGCAGCACGTAAATATTGGC1756341.044722291800714No Hit
ATCACATTGCCAGGGATTACC1478910.8796988308453909No Hit
ACAGATGATGAACTTATTG1460390.8686825943284584No Hit
ATCACATTGCCAGGGATTTCC1375960.8184611661899805No Hit
AACCCGTAGATCCGATCTTGTG1328460.7902067798749537No Hit
ACCTCAGAAGGTCTCACTTTT1328140.7900164345355685No Hit
AACCCGTAGATCCGATCTTGT1193190.7097442359416137No Hit
TGAGGTAGTAGATTGTATAGTT1079180.6419277604936939No Hit
AGGGGTAAAGCACTGTTACGTAT1058020.6293411749268315No Hit
AACCCGTAGATCCGAACTTGTG1032850.6143693243257953No Hit
TGAGGTAGTAGGTTGTATAGTT1000590.5951801347990003No Hit
TAGCAGCACGTAAATATTGGCGA981710.5839497597752593No Hit
TGAGGTAGTAGTTTGTGCTGTT926990.5514007067403485No Hit
TCCCTACACGACGCTCTCCCTAC876660.5214629538301319No Hit
TGAGGTAGTAGTTTGTACAGTT756030.44970870917367584No Hit
ATCACATTGCCAGGGATTTCCA619070.3682409039167063No Hit
TAGCTTATCAGACTGATGTTGA555910.33067149255550454No Hit
AACCCGTAGATCCGAACTTGT543560.3233253521135976No Hit
TGGCTCAGTTCAGCAGGAACA533790.31751387097048583No Hit
AACCCGTAGATCCGATCTTGTGA531780.316318264307471No Hit
AACCGGGCGGAAACACCA528030.3140876548615478No Hit
TTTGGCAATGGTAGAACTCACACT518260.3082761737184361No Hit
AACCCGTAGATCCGATCTTGTGT496950.2956003637737367No Hit
TAGTAGCACGTAAATATTGGCG475980.28312679575213445No Hit
ACTGGACTTGGAGTCAGAAGGC458340.2726340089185119No Hit
TAGGAGCACGTAAATATTGGCG455190.27076029698393644No Hit
TAGCTTATCAGACTGATGTTG445880.26522243726619127No Hit
AGCTACATTGTCTGCTGGGTTTC421120.2504944666312426No Hit
TGGCTCAGTTCAGCAGGAAC415640.2472348026942669No Hit
AAGCTGCCAGTTGAAGAACTGT396720.2359806345031026No Hit
TTCACAGTGGCTAAGTTCTGC373500.22216870081394643No Hit
TAGGATGGGGTGTGATAGGTGGC371420.22093145610794104No Hit
GGTATCGTAAACCGAAGATC343020.20403830723748298No Hit
TAGCACCATCTGAAATCGGTT318150.1892449053921206No Hit
AGCTCGGTCTGAGGCCCCTCAGT317270.18872145570881066No Hit
TTTGGCACTAGCACATTTTTGCT313700.18659791551629182No Hit
TGGCTCAGTTCAGCAGGA311630.18536661910214222No Hit
TAGGGGTAAAGCACTGTTACGTAT310960.18496808354780395No Hit
TTCAAGTAATCCAGGATAGGCTT299130.17793125428239837No Hit
TGTAAACATCCCCGACTGGAAGC278590.16571346281059526No Hit
AATCCCGAGTGTTGTTCTC269290.16018155138470583No Hit
CAAAGTGCTTACAGTGCAGGTAG254950.1516517008634957No Hit
TGGCAGTGTCTTAGCTGGTTGTT239350.14237236556845534No Hit
GAATCCCGAGTGTTGTTCTC234200.1393089952627209No Hit
TTCAAGTAATCCAGGATAGGC232740.1384405446517748No Hit
TAGGATGGGTGTGATAGGTGGC231710.1378278705906279No Hit
GCCGAAATAGCTCTCCCTACACG225850.134342171563132No Hit
TCCCTAC216470.1287626738023962No Hit
AAGCTGCCAGTTGAAGAACTG215430.1281440514493935No Hit
CAGTGGTTTTACCCTATGGTAG213440.1269603413700903No Hit
TGTAAACATCCCCGACTGGAAGCT211950.12607404588357682No Hit
AAGGAGCTCACAGTCTATTGAG210180.1250211982251011No Hit
CAGCAGCAATTCATGTTTTGA208870.12424197199199193No Hit
CCCCCGCGGGGGCGCGCCGGCG208440.12398619544219275No Hit
TATGGCACTGGTAGAATTCACT208020.12373636718424935No Hit
ATCACATTGCCAGGGATTACCACT207800.12360550476342186No Hit
ACCTCAGAAGGTCTCACTTT205070.12198162108678981No Hit
AGCAGCATTGTACAGGGCTATGA204960.12191618987637605No Hit
GGTGTCGTAAACCGAAGATC200320.11915618245528714No Hit
TAGCAGCACGTAAATATTGGCA196280.11675307254554591No Hit
ATCCCGAGTGTTGTTCTC190680.11342202910630068No Hit
AGTAAGGTCAGCTAAATAAGCTA190230.1131543559727899No Hit
ACACGACGCTCTTCCGATCTCAC185870.11056090072366323No Hit
CCTACACGACGCTCTCCCTACACG185850.11054900413995164No Hit
ATCACATTGCCAGGGATTTC183630.10922848334796514No Hit
TGGCTCAGTTCAGCAGGAACAGT182440.10852063661712552No Hit
TAGCAGCACATCATGGTTTACA181390.10789606597226704No Hit
CACCCGTAGAACCGACCTTGCG179360.10668856272554063No Hit
NAGCAGCACGTAAATATTGGCG174900.10403562455785605No Hit
ACCATCGACCGTTGATTGTACC173900.10344079537227653No Hit
TGGCTCAGTTCAGCAGGAACAG169340.10072837428603397No Hit
CAAAGTGCTTACAGTGCAGGTAGT168190.10004432072261751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT15650.03072.146226
CGAGCTA2550.02870.588128
CGGGCTA18900.02640.692427
CGCTCTA201.4188117E-92495.454328
ACGAGCT3100.02361.2927
TCCGGGC4100.02164.992225
CGACGGA3800.02141.25325
CGACGGT2800.02113.444625
ACGACGT3050.02080.343324
GGGCTAA27100.01995.135728
GGACTAA3300.01966.115528
TCGCCAC700.01901.298628
CGTTCTA1050.01901.298628
CGGACTA6200.01878.29928
CGACGTG2500.01775.293625
ACGACGA3500.01775.102324
ATTACGG700.01699.56624
ACGGACT7350.01584.415527
ACGTGCT4150.01523.329627
ACGACGG9900.01522.170324
Produced by FastQC (version 0.11.5)