FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15878337
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG162970910.263725980875705No Hit
AATCGTACAGGGTCATCCACTT5104563.214795101023489No Hit
TTAATATCGGACAACCATTGT2852581.7965231497479868No Hit
TTCAAGTAATTCAGGATAGGTT2794911.7602032253125752No Hit
TAGCTTATCAGACTGATGTTGAC2359051.4857034461480445No Hit
TAGCAGCACGTAAATATTGGCGT2349701.4798149201644983No Hit
GGTTTCGTAAACCGAAGATC2020791.2726710612074803No Hit
TATACAAGGGCAAGCTCTCTGT1636581.0306998774493827No Hit
TTCAAGTAATCCAGGATAGGCT1414140.8906096400397597No Hit
ATCATAGAGGAAAATCCACGT1405210.8849856253838169No Hit
AACCGGGCGGAAACACCA1245900.7846539596684463No Hit
TTGTACATGGTAGGCTTTCATT1200540.7560867362873077No Hit
TAGTAGACCGTATAGCGTACG1182470.7447064513116204No Hit
TCCCTACACGACGCTCTCCCTACACG985350.620562468223215No Hit
CACAGATGATGAACTTATTG936760.5899610267750331No Hit
AATCATACACGGTTGACCTATT851940.5365423343767045No Hit
AGGGGTAAAGCACTGTTACGTAT825670.5199977806240036No Hit
TGTCTTGCAGGCCGTCATGC796750.501784286351902No Hit
ATAGTAGACCGTATAGCGTACG794540.5003924529376093No Hit
AACATAGAGGAAATTCCACGT769840.4848366677190439No Hit
AACCCGTAGATCCGATCTTGTG753700.4746718752725805No Hit
TGTCTTGCAGGCCGTCATGCA740990.46666725866820935No Hit
TCGGATCCGTCTGAGCTTGGCT716960.4515334319960585No Hit
TATGTCTGCTGACCATCACC711300.44796882696216866No Hit
TAGCAGCACGTAAATATTGGC707650.4456700975675224No Hit
TCCCTACACGACGCTCTCCCTAC692250.4359713488887407No Hit
ACAGATGATGAACTTATTG685240.4315565288732693No Hit
TTTGGCAATGGTAGAACTCACACT665970.4194204972472873No Hit
TGGTAGACTATGGAACGTAGG646130.40692548596241535No Hit
TCGTACAGGGTCATCCACTTT621300.39128782819006797No Hit
TGAAACATACACGGGAAACCTCT598730.37707349327577566No Hit
AATTGCACGGTATCCATCTGTA574020.36151141016845784No Hit
GAATGTTGCTCGGTGAACCCCT517670.32602280704837033No Hit
AACCCGTAGATCCGATCTTGT476420.3000440159444909No Hit
TAGCTTATCAGACTGATGTTGA456170.2872907912207683No Hit
TTTGGCACTAGCACATTTTTGCT436850.275123270151024No Hit
ACCTCAGAAGGTCTCACTTTT434660.27374403251423624No Hit
AGCAGCATTGTACAGGGCTATGA434280.2735047127416429No Hit
ATCGTACAGGGTCATCCACTTT426760.2687687003997963No Hit
AATCGTACAGGGTCATCCACTTT391830.24677017498746875No Hit
TAGCTTATCAGACTGATGTTG351590.22142747064758736No Hit
TGGAGTGTGACAATGGTGTTT348380.21940584835804908No Hit
TAAAGTGCTGACAGTGCAGATA339630.21389519569965043No Hit
CTGACCTATGAATTGACAGCC327270.20611100520161527No Hit
TAGCAGCACGTAAATATTGGCGA314710.19820085692853098No Hit
GGTATCGTAAACCGAAGATC313830.19764664271831492No Hit
GTTGATGATGATGTTCTGAAA300670.18935862112008328No Hit
CAAAGTGCTGTTCGTGCAGGTAG297770.18753223338187117No Hit
TGGAGTGTGACAATGGTGTTTG295250.18594516541625233No Hit
AATCGTACAGGGTCATCCACT279810.17622122518246086No Hit
CAAAGTGCTTACAGTGCAGGTAG274750.1730344934737183No Hit
AATAATACATGGTTGATCTTT261010.1643811943278443No Hit
AATCATACAGGGACATCCAGTT259820.16363174556630206No Hit
AACCCGTAGATCCGATCTTGTGA247920.15613725795087988No Hit
TGGTTTACCGTCCCACATACAT245290.15448091320898405No Hit
TCGAGGAGCTCACAGTCTAGTA245110.15436755121143983No Hit
TATGGCACTGGTAGAATTCACT244180.15378184755746147No Hit
TCGGATCCGTCTGAGCTTGG239540.15085962717632206No Hit
AAGCTGCCAGTTGAAGAACTGT236280.1488065154430215No Hit
ATATAATACAACCTGCTAAGTG229460.1445113553138468No Hit
TAGCACCATCTGAAATCGGTT229300.1444105890938075No Hit
TGTAACAGCAACTCCATGTGGA227810.14347220366969163No Hit
TGAGGTAGTAGATTGTATAGTT224920.14165211382023193No Hit
TGTCTTGCAGGCCGTCATGCAGG224130.14115458060878794No Hit
TAGGGGTAAAGCACTGTTACGTAT224100.14113568694253056No Hit
TGTCTTGCAGGCCGTCATGCAG222870.14036104662597854No Hit
TGAGGTAGTAGGTTGTATAGTT220770.13903848998796284No Hit
TAGGATGGGGTGTGATAGGTGGC216920.13661380281826743No Hit
CATTGCACTTGTCTCGGTCTGA213250.13430247764611622No Hit
AATCCCGAGTGTTGTTCTC211620.13327592177946596No Hit
TGAAGGTCTACTGTGTGCCAGG208700.13143693826374891No Hit
TAAAGTGCTGACAGTGCAGAT205590.1294782948617352No Hit
CGAATGTTGCTCGGTGAACCCCT202410.1274755662384543No Hit
AACCCGTAGATCCGATCTTGTGT200160.12605854126915178No Hit
TCCCTAC199610.12571215738776673No Hit
GTTTCCGTAGTGTAGTGGTTATC198200.12482415507367049No Hit
GGTGTCGTAAACCGAAGATC196290.12362125832195148No Hit
TGTAAACATCCCCGACTGGAAGCT195370.12304185255672556No Hit
ACACGACGCTCTTCCGATCTCAC192160.1210202302671873No Hit
GAATCCCGAGTGTTGTTCTC188710.11884745864759012No Hit
TAGTAGCACGTAAATATTGGCG183470.11554736494130337No Hit
AAGGAGCTCACAGTCTATTGAG183090.11530804516871004No Hit
GAAAATGTTTAG182860.11516319372740356No Hit
AGCAGCATTGTACAGGGCTATG182730.11508132117362166No Hit
TCGAGGAGCTCACAGTCTAGT181970.11460268162843501No Hit
TGTAAACATCCCCGACTGGAAGC179910.11330531654542915No Hit
AGTAAGGTCAGCTAAATAAGCTA178580.11246769734135256No Hit
AAAGGTTCGTTTGTAAAAAA178090.11215910079248224No Hit
ATCGTACAGGGTCATCCACTT176440.11111994914832705No Hit
GCCCGGATAGCTCAGTCGG174820.11009969117042925No Hit
TAAAGTGCTTATAGTGCAGGTAG172620.108714155644889No Hit
CAAAGTGCTTACAGTGCAGGTAGT171030.10771279133324856No Hit
TAGGAGCACGTAAATATTGGCG166990.1051684442772565No Hit
AAAGGTTCGTTTGTAAAAA165750.104387506071952No Hit
CAAAGAATTCTCCTTTTGGGCT165330.10412299474434886No Hit
GAACGGCTTCATACAGGAGT160790.10126375325073401No Hit
TTCAAGTAATCCAGGATAGGC159280.1003127720491132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT16800.02943.36426
CGGGCTA20500.02540.769327
CGAGCTA2050.02331.718828
TCCGGGC5400.02044.877125
GGGCTAA29400.01951.0328
TCGCCAC1100.01947.971928
GGACTAA3300.01947.971928
ACGAGCT2700.01770.37927
ACGGTCT5550.01514.639326
TTCGCCA1500.01428.512727
CGGACTA5350.01386.407128
ACGACGA1950.01317.023824
CCCGGGC15050.01264.684425
CGGCTAA2700.01220.95128
ACGACGT1700.01208.56324
CGACGGA2800.01193.485625
ACGGACT6200.01169.749927
CGACGGT1250.01162.351225
CGGCCTA2850.01156.690627
GGCCTAA3300.01148.80428
Produced by FastQC (version 0.11.5)