FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16791369
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG271105216.14550904098409No Hit
TAGCTTATCAGACTGATGTTGAC5316343.166114686658366No Hit
TAGCAGCACGTAAATATTGGCGT4898392.9172070484544768No Hit
AACCCGTAGATCCGAACTTGTG4046902.4101072402137076No Hit
TTCAAGTAATTCAGGATAGGTT3587752.1366631869027475No Hit
TTCAAGTAATCCAGGATAGGCT2739881.6317192481446867No Hit
CACAGATGATGAACTTATTG2586811.5405593194932468No Hit
ACAGTAGTCTGCACATTGGTT2404621.432057147931178No Hit
AACCCGTAGATCCGAACTTGT1972561.1747463830971734No Hit
ACAGATGATGAACTTATTG1488840.8866698123303705No Hit
TAGCAGCACGTAAATATTGGC1324180.7886075280699268No Hit
TAGCTTATCAGACTGATGTTGA1177490.7012471704957469No Hit
ATCACATTGCCAGGGATTTCC1115170.6641328649260224No Hit
GGTTTCGTAAACCGAAGATC1069360.6368509917208061No Hit
TGAGATGAAGCACTGTAGCTC952900.5674939309594114No Hit
TAGCTTATCAGACTGATGTTG855360.5094045637374773No Hit
TACAGTAGTCTGCACATTGGTT779390.46416108180339555No Hit
TGAGAACTGAATTCCATGGGTT766090.45624034585863726No Hit
TGAGGTAGTAGGTTGTATAGTT706600.42081142996738385No Hit
AACCCGTAGATCCGAACTTGTGT623380.37125025362732483No Hit
ATCACATTGCCAGGGATTTCCA562930.33524961544231446No Hit
ACCTCAGAAGGTCTCACTTTT555660.33092001015521727No Hit
AGGGGTAAAGCACTGTTACGTAT540020.3216057011194263No Hit
AACCCGTAGATCCGATCTTGTG537570.320146618182234No Hit
CCCAGTGTTCAGACTACCTGTTC520670.310081923635887No Hit
TAGGATGGGGTGTGATAGGTGGC470880.28042978508780314No Hit
TGGCTCAGTTCAGCAGGAACA469240.2794530928359683No Hit
ATCACATTGCCAGGGATTACC458300.2729378408633626No Hit
TAGCAGCACGTAAATATTGGCGA447120.26627965831731765No Hit
TAGCTTATCAGACTGATGTTGACA417260.24849671280525132No Hit
CCCAGTGTTCAGACTACCTGTT408020.24299388572784028No Hit
CAGTGCAATGATGAAAGGGCATT392780.2339177943144481No Hit
TAGCTTATCAGACTGATGTTGACC383710.22851620972655654No Hit
AGCTACATTGTCTGCTGGGTTTC379940.22627100863544836No Hit
AAGGAGCTCACAGTCTATTGAG374220.2228644966351463No Hit
TCCCTACACGACGCTCTCCCTACACG372830.22203669039731067No Hit
TTTGGCAATGGTAGAACTCACACT354360.2110369916830486No Hit
AGCTCGGTCTGAGGCCCCTCAGT354080.21087023934736945No Hit
ACAGTAGTCTGCACATTGGTTA345400.2057009169413167No Hit
CAGTGCAATGTTAAAAGGGCATT343230.20440858633980352No Hit
AACCCGTAGATCCGATCTTGT327700.1951597871501722No Hit
TCGAGGAGCTCACAGTCTAGTA321240.19131257254843245No Hit
AATCGTACAGGGTCATCCACTT319760.19043116734555712No Hit
TGAGATGAAGCACTGTAGCTCT319760.19043116734555712No Hit
TTCAAGTAATCCAGGATAGGCTT319180.19008575179307893No Hit
AGCAGCATTGTACAGGGCTATGA308000.183427569247034No Hit
TAGGTAGTTTCCTGTTGTTGGG297250.1770254706450677No Hit
ACCATCGACCGTTGATTGTACC292160.17399415140004368No Hit
TAGGATGGGTGTGATAGGTGGC287990.17151073268653674No Hit
TGGCTCAGTTCAGCAGGA283790.16900944765134993No Hit
TTCAAGTAATCCAGGATAGGC283220.16866998753943171No Hit
AAAGGTTCGTTTGTAAAAAA274640.16356021953897862No Hit
AACCCGTAGATCCGAACTTGTGA271260.16154728062970922No Hit
TGAGGTAGTAGATTGTATAGTT268220.15973682669947878No Hit
CAGCAGCAATTCATGTTTTGA266680.15881968885324357No Hit
TGAGATGAAGCACTGTAGCT260220.15497247425150384No Hit
TAAAGTGCTGACAGTGCAGATA259450.15451390532838627No Hit
AGGCAAGATGCTGGCATAGCTG258260.15380520790175No Hit
ACTGGACTTGGAGTCAGAAGGC257660.1534478814681519No Hit
AAAGGTTCGTTTGTAAAAAAA254990.15185777883864024No Hit
TTTGGCACTAGCACATTTTTGCT249430.14854655388729768No Hit
CAACACCAGTCGATGGGCTGTC239660.14272808846020835No Hit
TAGGTAGTTTCCTGTTGTTGGGA238900.14227547497765072No Hit
TAGGTAGTTTCATGTTGTTGGG236020.14056030809637976No Hit
TGGCTCAGTTCAGCAGGAAC234580.13970272465574426No Hit
AAAGGTTCGTTTGTAAAAA231600.13792800336887362No Hit
TAGCACCATCTGAAATCGGTT229240.13652251939672103No Hit
AACCGGGCGGAAACACCA226190.13470611002593058No Hit
TCGAGGAGCTCACAGTCTAGT225700.13441429343849212No Hit
TAGCTTATCAGACTGATGTTGACT216750.1290841741373202No Hit
AGGCAAGATGCTGGCATAGCT214260.12760126943788802No Hit
CAAAGTGCTGTTCGTGCAGGTAG209020.12448061858446444No Hit
TAGGGGTAAAGCACTGTTACGTAT202820.12078824543728388No Hit
GGTATCGTAAACCGAAGATC201100.11976390966096928No Hit
TAAAGTGCTGACAGTGCAGAT194140.11561892303123111No Hit
TAAAGGTTCGTTTGTAAAAA193040.11496382456963455No Hit
GAATCCCGAGTGTTGTTCTC192870.11486258208011509No Hit
TGGCTCAGTTCAGCAGGAACAGT192030.11436232507307771No Hit
AGCAGCATTGTACAGGGCTATGT190090.1132069696044438No Hit
AATGACACGATCACTCCCGTTGAGT188360.11217667838756924No Hit
CAGTAGTCTGCACATTGGTT184870.11009822963214018No Hit
ATCACATTGCCAGGGATTTC184090.10963370526846261No Hit
AATCCCGAGTGTTGTTCTC183280.10915131458310516No Hit
TACCCTGTAGATCCGAATTTGT182340.10859150317046812No Hit
TAAAGGTTCGTTTGTAAAAAA181170.10789471662495179No Hit
AAGCTGCCAGTTGAAGAACTGT180550.10752547931023373No Hit
AAAGGTTCGTTTGTAAAAAAAA178260.10616168342200091No Hit
AGGCAAGATGCTGGCATAGC176780.10528027821912556No Hit
AACATTCATTGCTGTCGGTGGGTT175880.10474428856872837No Hit
CCCAGTGTTTAGACTATCTGTTC175760.10467282328200875No Hit
TGAGGTAGTAGTTTGTGCTGTT172920.10298147816297765No Hit
ATCACATTGCCAGGGATTACCACT168630.1004265941627511No Hit
AGTAAGGTCAGCTAAATAAGCTA168080.10009904493195285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA2900.02218.943428
CCGGGCT5700.02197.176826
CTATATT202.434832E-62075.785628
CGGGCTA10000.01859.814627
ACGGACT3500.01848.263127
GGGCTAA15800.01813.02828
CCTATTT900.01795.542426
TCGCCAC600.01729.821428
GGACTAA1350.01691.38128
AGATTAC900.01614.528
TCGCCTA1650.01509.662428
CGGCTAA3650.01478.641828
GACATCT850.01465.260528
TCCTATT1000.01409.254225
TCACTTA157.95469E-61399.319222
TTCGCCT2800.01371.757727
ATTACGG1550.01283.201724
ACGGTCT4800.01263.46127
CATCAGA7550.01259.929225
ATTCGGG750.01223.976924
Produced by FastQC (version 0.11.5)