FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19942616
Sequences flagged as poor quality0
Sequence length0-34
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG8842194.433816506319934No Hit
TCCCTACACGACGCTCTCCCTACACG3820841.9159171494853033No Hit
GGTTTCGTAAACCGAAGATC3085411.5471440657534596No Hit
TCCCTACACGACGCTCTCCCTAC1964830.9852418559330431No Hit
AACCGGGCGGAAACACCA1722240.8635978349079177No Hit
TTCAAGTAATCCAGGATAGGCT1569280.7868977670732866No Hit
TAGCTTATCAGACTGATGTTGAC1515080.7597197880157749No Hit
TGAGATGAAGCACTGTAGCTC1417040.7105587351228144No Hit
AGGGGTAAAGCACTGTTACGTAT1360620.6822675620891462No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1300660.6522012959583637No Hit
TAGCAGCACGTAAATATTGGCGT1201980.6027193222794842No Hit
AACCCGTAGATCCGAACTTGTG1153480.5783995439715632No Hit
ACAGATGATGAACTTATTG1093120.5481327023495814No Hit
ATCACATTGCCAGGGATTTCC1004410.5036500727888458No Hit
ACTGGACTTGGAGTCAGAAGGC834230.4183152300580827No Hit
CAGCAGCAATTCATGTTTTGA824990.4136819362113777No Hit
GCATTGGTGGTTCAGTGGTAGAATT797760.40002775964798193No Hit
GAATCCCGAGTGTTGTTCTC786050.39415591214312107No Hit
GTTTCCGTAGTGTAGTGGTTATC780330.39128768261897034No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC769520.38586712996930794No Hit
AATCCCGAGTGTTGTTCTC689960.34597266476975735No Hit
ACACGACGCTCTTCCGATCTCAC675380.3386616881155411No Hit
CACAGATGATGAACTTATTG667780.3348507537827535No Hit
TCCCATATGGTCTAGCGGTTAGGATT665270.33359214257547753No Hit
TTCAAGTAATTCAGGATAGGTT646260.3240597923562285No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA642200.3220239511205551No Hit
TGCGGGGCTAGGGCTAACAGCA598440.3000809923833463No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCT587050.29436960527144485No Hit
GCCCGGATAGCTCAGTCGGTAGAGC572470.2870586286172286No Hit
ATCACATTGCCAGGGATTACC555190.27839376739741667No Hit
AGCTACATTGTCTGCTGGGTTTC549650.27561579684430565No Hit
GAAAATGTTTAG532450.26699105072273366No Hit
ATCCCGAGTGTTGTTCTC515610.2585468225432411No Hit
TTGGAATCCCGAGTGTTGTTCTC510550.2560095425795693No Hit
GGCTTGGTCTAGGGGTATGATT506170.25381324095093644No Hit
GGTTCCATAGTGTAGTGGTTATC504630.2530410253098189No Hit
GGTATCGTAAACCGAAGATC499580.2505087597334271No Hit
ACCTCAGAAGGTCTCACTTTT477110.23924143151530372No Hit
TCCCTAC472630.23699498601387098No Hit
CAGTGCAATGATGAAAGGGCATT457420.22936810296101573No Hit
ACCATCGACCGTTGATTGTACC450470.22588310380142707No Hit
CACGACGCTCTTCCGATCTCC440440.22085367335960338No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC435080.21816596177753209No Hit
AAGCTGCCAGTTGAAGAACTGT433390.21731853032721485No Hit
TTTGGCAATGGTAGAACTCACACT427330.2142798116355447No Hit
TGGCTCAGTTCAGCAGGAAC418150.20967660411251965No Hit
AAGTTTCTCTGAACGTGTAGAGC413630.207410101061967No Hit
ATCACATTGCCAGGGATTTCCA408670.20492296497109505No Hit
CAGTGCAATGTTAAAAGGGCATT401730.20144298019878637No Hit
TGGCTCAGTTCAGCAGGAACA394190.19766213218967862No Hit
TGGAATCCCGAGTGTTGTTCTC388870.19499447815672727No Hit
AACCCGTAGATCCGAACTTGT383370.19223656515273624No Hit
TGGCTCAGTTCAGCAGGA371250.18615912776939594No Hit
TACACGACGCTCTTCCGATCTCTAC366580.18381740890964354Illumina Single End PCR Primer 1 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATT364330.18268917177164723No Hit
AGCTCGGTCTGAGGCCCCTCAGT355480.17825143902886162No Hit
TAGCAGCACGTAAATATTGGC351920.17646631715718739No Hit
GTTGGAATCCCGAGTGTTGTTCTC350770.17588966261998928No Hit
CACCCGTAGAACCGACCTTGCG345820.17340754091639732No Hit
AACATTCAACGCTGTCGGTGAGT345650.1733222963326376No Hit
TCGAGGAGCTCACAGTCTAGT342640.1718129657613625No Hit
TCCCACATGGTCTAGCGGTTAGGATT336340.16865390177497275No Hit
AGGCAAGATGCTGGCATAGCTG332210.16658295982833948No Hit
TCCCGAGTGTTGTTCTC329420.165183945777224No Hit
GCCCGGATAGCTCAGTCGG329360.1651538594535441No Hit
AGCAGCATTGTACAGGGCTATGA324800.16286729885387152No Hit
GGCTGTAGGAATACTTTT319070.15999405494244084No Hit
GTTTCCGTAGTGTAGTGGTCATC317850.15938229969428283No Hit
TAGCTTATCAGACTGATGTTG317620.1592669687868432No Hit
TGTAAACATCCTCGACTGGAAGCT315850.1583794222382861No Hit
TCGAGGAGCTCACAGTCTAGTA314480.15769245118092834No Hit
TGGAGAGAAAGGCAGTTCCTGA299650.15025611484471246No Hit
CCTACACGACGCTCTCCCTACACG297990.14942372655623515No Hit
TCCCTGGTCTAGTGGTTAGGATT297480.149167992804956No Hit
GCCGAAATAGCTCTCCCTACACG297420.1491379064812761No Hit
CGTTGGAATCCCGAGTGTTGTTCTC293010.14692656169080326No Hit
TACCCTGTAGAACCGAATTTGT292220.14653042509568454No Hit
TAGCTTATCAGACTGATGTTGA288840.14483556219505003No Hit
CAGTGGTTTTACCCTATGGTAG285450.14313568490713555No Hit
AATGACACGATCACTCCCGTTGAGT281910.1413605918100213No Hit
AGTAAGGTCAGCTAAATAAGCTA277950.13937489444714776No Hit
AACATTCATTGCTGTCGGTGGGTT275880.13833691628019112No Hit
GCATGGGTGGTTCAGTGGTAGAATT268600.13468644234036298No Hit
GGTGTCGTAAACCGAAGATC261790.13127164460269405No Hit
CTCAGGATGTGAATCTGCGC244860.12278228693768159No Hit
CAGTGCAATGTTAAAAGGGCATA243490.12209531588032382No Hit
AGCGAACCAACAGCCAAGGGA242140.12141837359752602No Hit
AAGCTGCCAGTTGAAGAACTG239880.12028512207224969No Hit
TGCGGGGCTAGGGCTAACAGC238060.11937250358729266No Hit
CTTTCAGTCGGATGTTTGCAGC236020.11834956858217598No Hit
AGGCAAGATGCTGGCATAGC234400.11753723784281862No Hit
AGCAGAGTGGCGCAGCGGAAGCGTAC230750.11570698648562455No Hit
CAGTGCAATGATGAAAGGGCATA229760.11521056214490617No Hit
AGGCAAGATGCTGGCATAGCT226150.11340036833683205No Hit
AAAAGCGTAGTCGATGGACA224040.11234233262075548No Hit
TAGGATGGGGTGTGATAGGTGGC219140.10988528285356344No Hit
TAGCAGCACGTAAATATTGGCGA216820.10872194500460722No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGC213960.10728783024253187No Hit
CAAAGTGCTTACAGTGCAGGTAG209590.10509654300117899No Hit
TAGGTAGTTTCATGTTGTTGGG207150.10387303250486296No Hit
AAAGCGTAGTCGATGGACA207100.10384796056846304No Hit
TACACGACGCTCTTCCGATCCCTAC206580.10358721242990389No Hit
TTCAAGTAATCCAGGATAGGCTT205550.10307073054006556No Hit
TACCCTGTAGATCCGAATTTGT205160.10287516943614618No Hit
GCGAACCAACAGCCAAGGGA202590.10158647190519038No Hit
TAGGGGTAAAGCACTGTTACGTAT202500.10154134241967053No Hit
GCCCGGCTAGCTCAGTCGGTAGAGC201180.10087944329871268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTA5700.02130.087428
CGGCTAA9850.01749.552928
TCGCCAC2150.01700.234928
CCGGGCT7300.01377.073926
ATGAGAC13750.01329.274526
CGGGCTA13750.01281.800427
AATATTT9100.01262.497928
TACACCC750.01218.501727
ACGGCTA15050.01119.032127
CGCTAAT3150.01036.14128
CTACACC1400.01025.779426
GCTGGGC18550.0994.102825
CTAATAT11450.0991.9804726
CATGAGA18800.0963.9715625
ACGCTAA3450.0946.041727
CTGCGCA2500.0939.987127
CGCCAAC3100.0926.5105628
TTCGCCA4050.0902.593927
CACGGCT3300.0870.3583426
GCATGAG23400.0867.9732724
Produced by FastQC (version 0.11.5)