FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002441066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10887152
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG218966620.112385681765073No Hit
TAGCAGCACGTAAATATTGGCGT3679263.3794513018648034No Hit
TGAGGTAGTAGTTTGTGCTGTT3395593.118896475405138No Hit
GGTTTCGTAAACCGAAGATC2668402.4509623820811908No Hit
TGAGGTAGTAGATTGTATAGTT2148301.9732433238738651No Hit
AACCCGTAGATCCGAACTTGTG2125441.9522460970509092No Hit
TTCAAGTAATTCAGGATAGGTT2044391.87780054875692No Hit
TTCAAGTAATCCAGGATAGGCT1761861.6182928280968245No Hit
CACAGATGATGAACTTATTG1421781.3059246348356301No Hit
AACCGGGCGGAAACACCA1088250.9995727073526667No Hit
TGAGGTAGTAGGTTGTATAGTT1034400.9501107360308738No Hit
TAGCAGCACGTAAATATTGGC1005830.923868795071475No Hit
AACCCGTAGATCCGAACTTGT996570.9153633567346171No Hit
TCCCTACACGACGCTCTCCCTACACG936020.8597473425557023No Hit
TAGCTTATCAGACTGATGTTGAC924910.8495426535791913No Hit
ATCACATTGCCAGGGATTTCC862540.7922549441764017No Hit
ACAGATGATGAACTTATTG799530.734379385903678No Hit
TGAGGTAGTAGTTTGTACAGTT629860.5785351394010114No Hit
TAGCAGCACGTAAATATTGGCGA487460.44773876584069006No Hit
ATCACATTGCCAGGGATTACC462890.4251708803183789No Hit
GGTATCGTAAACCGAAGATC435540.40004952626729195No Hit
TCCCTACACGACGCTCTCCCTAC419120.384967528698047No Hit
ATCACATTGCCAGGGATTTCCA416540.38259776294112546No Hit
AGGGGTAAAGCACTGTTACGTAT409940.37653557146993083No Hit
AGCTACATTGTCTGCTGGGTTTC324140.29772708234440004No Hit
AGTAAGGTCAGCTAAATAAGCTA308700.28354522835724166No Hit
TGAGGTAGTAAGTTGTATTGTT306850.28184597771758857No Hit
ACCTCAGAAGGTCTCACTTTT286930.2635491816408919No Hit
TAGGATGGGGTGTGATAGGTGGC273970.2516452420247279No Hit
ATCACATTGCCAGGGATTACCACT260060.23886871424225548No Hit
TAGTAGCACGTAAATATTGGCG255300.23449658827212114No Hit
GGTGTCGTAAACCGAAGATC246770.22666166505253163No Hit
TAGGAGCACGTAAATATTGGCG238360.21893696349605482No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT228320.20971508434896474No Hit
TGGCTCAGTTCAGCAGGAACA220620.20264252763257093No Hit
TTCAAGTAATCCAGGATAGGCTT215780.19819692055369487No Hit
AACCCGTAGATCCGAACTTGTGT212240.19494538149187227No Hit
TAGCTTATCAGACTGATGTTGA208300.19132643688634088No Hit
AATCCCGAGTGTTGTTCTC206690.1898476295729131No Hit
TGGCTCAGTTCAGCAGGAACAGT198090.18194841038317458No Hit
AAGCTGCCAGTTGAAGAACTGT196910.1808645640292337No Hit
AACCCGTAGATCCGAACTTGTGA186600.17139468614014022No Hit
TTCAAGTAATCCAGGATAGGC184530.16949336245144736No Hit
TTAGGTAGTAGTTTGTGCTGTT174030.1598489669290922No Hit
ACCATCGACCGTTGATTGTACC173940.15976630068175773No Hit
TGGCTCAGTTCAGCAGGAAC173470.15933459916789994No Hit
TAGCTTATCAGACTGATGTTG172650.15858141780329696No Hit
AGAGGTAGTAGGTTGCATAGTT169800.15596365330437198No Hit
TAGGATGGGTGTGATAGGTGGC165880.15236307897602605No Hit
AAGGAGCTCACAGTCTATTGAG161790.1486063572916039No Hit
TGTTTCGTAAACCGAAGATC152500.14007336353896777No Hit
GTTTCCGTAGTGTAGTGGTTATC148470.13637175268610194No Hit
GAATCCCGAGTGTTGTTCTC148290.136206420191433No Hit
TGGCTCAGTTCAGCAGGA145740.13386420985028957No Hit
TTCACAGTGGCTAAGTTCTGC142080.13050244912535436No Hit
ATCCCGAGTGTTGTTCTC133700.12280530298465567No Hit
TAGCAGCACGTAAATATTGGCA131270.1205733143066249No Hit
AGCTACATTGTCTGCTGGGTTT129040.11852502840044851No Hit
GCGCCGCTGGTGTAGTGGTA128940.11843317701452133No Hit
GCCCGGATAGCTCAGTCGG128100.11766162537273292No Hit
ACACGACGCTCTTCCGATCTCAC120360.11055232810196827No Hit
TTAGGTAGTAGATTGTATAGTT119400.10967055479706722No Hit
TCCCATATGGTCTAGCGGTTAGGATT118510.10885307746231522No Hit
AAGCTGCCAGTTGAAGAACTG117490.10791619332585786No Hit
TCCCTAC112250.10310318070327298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)