FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14193628
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG182732412.874255968946066No Hit
TGAGGTAGTAGTTTGTGCTGTT4073972.870280945787786No Hit
AACCCGTAGATCCGAACTTGTG3974132.7999395221574073No Hit
TAGCAGCACGTAAATATTGGCGT3631372.558450876689174No Hit
GGTTTCGTAAACCGAAGATC2825601.9907524700520545No Hit
TAGCTTATCAGACTGATGTTGAC2709661.9090679282280754No Hit
CACAGATGATGAACTTATTG1598451.1261743650037892No Hit
TGAGGTAGTAGATTGTATAGTT1351100.9519060243089363No Hit
TTCAAGTAATTCAGGATAGGTT1292570.9106692101554302No Hit
AGGGGTAAAGCACTGTTACGTAT1180220.8315139723261734No Hit
AACCCGTAGATCCGAACTTGT1165830.8213756201022037No Hit
TTCAAGTAATCCAGGATAGGCT1124130.7919962394392752No Hit
ACAGATGATGAACTTATTG1070410.7541482699137951No Hit
TGAGGTAGTAGGTTGTATAGTT936850.6600497068120991No Hit
TCCCTACACGACGCTCTCCCTACACG852560.6006639035488319No Hit
ATCACATTGCCAGGGATTTCC793310.558919819513376No Hit
TAGCAGCACGTAAATATTGGC765940.5396365185842548No Hit
AACCCGTAGATCCGAACTTGTGT649410.45753629727367806No Hit
AAAGGTTCGTTTGTAAAAAA591900.41701811545293427No Hit
AGCTACATTGTCTGCTGGGTTTC575650.4055693160339273No Hit
AAAGGTTCGTTTGTAAAAA568790.40073616132534967No Hit
TAGCTTATCAGACTGATGTTGA560130.3946348319118974No Hit
ATCACATTGCCAGGGATTTCCA551630.38864622913887836No Hit
TGAGGTAGTAGTTTGTACAGTT541080.38121331628530775No Hit
TCCCTACACGACGCTCTCCCTAC523190.36860906880185956No Hit
AAAGGTTCGTTTGTAAAAAAA518020.3649665892328586No Hit
TAGCTTATCAGACTGATGTTGACC502140.3537784701698537No Hit
TAGCTTATCAGACTGATGTTG482350.33983559383126005No Hit
GGTATCGTAAACCGAAGATC463260.3263858965445621No Hit
TAGGATGGGGTGTGATAGGTGGC425730.2999444539479265No Hit
TAAAGGTTCGTTTGTAAAAA406830.28662861954674307No Hit
AGCTACATTGTCTGCTGGGTTT361340.25457902658855086No Hit
AAAGGTTCGTTTGTAAAAAAAA359800.25349403267438037No Hit
AGTAAGGTCAGCTAAATAAGCTA347270.24466612764544765No Hit
ACCATCGACCGTTGATTGTACC342970.24163659918380276No Hit
TAGCAGCACGTAAATATTGGCGA340730.24005842621773657No Hit
AGGCAAGATGCTGGCATAGCTG339440.2391495676792431No Hit
TAAAGGTTCGTTTGTAAAAAA336950.2373952593374999No Hit
CTTCGTGATCGATGTGGTGACG333200.23475322870234447No Hit
TGTAAACATCCTCGACTGGAAGCT320480.22579146078789722No Hit
AACCGGGCGGAAACACCA316080.22269147817598148No Hit
TAGGGGTAAAGCACTGTTACGTAT314600.22164875675197349No Hit
GGTGTCGTAAACCGAAGATC306390.21586447101473988No Hit
ATCACATTGCCAGGGATTACC291460.20534566637930768No Hit
TAAAGGTTCGTTTGTAAAAAAA269270.18971189043421455No Hit
TAGGATGGGTGTGATAGGTGGC266250.18758417509603606No Hit
AAGGTTCGTTTGTAAAAAAA254640.1794044482495948No Hit
GGCTCAGTAGCCAGTGTAGATCC254110.1790310412531595No Hit
CTTTCAGTCGGATGTTTGCAGC249940.17609310318686666No Hit
AAGGTTCGTTTGTAAAAAA242110.17057654322066212No Hit
ACCTCAGAAGGTCTCACTTTT239480.16872359906853976No Hit
AATCCCGAGTGTTGTTCTC236080.16632815795933217No Hit
GAATCCCGAGTGTTGTTCTC227260.1601141019054466No Hit
TGGCTCAGTTCAGCAGGAACA224480.15815547652791803No Hit
TGAGGTAGTAAGTTGTATTGTT221880.15632366862087693No Hit
CAGTGCAATGTTAAAAGGGCATT220160.15511185723621895No Hit
ACTGGACTTGGAGTCAGAAGGC218510.15394936375675056No Hit
TTCACAGTGGCTAAGTTCCG217380.1531532318586904No Hit
AGGCAAGATGCTGGCATAGCT215090.15153983181748878No Hit
TTCAAGTAATCCAGGATAGGCTT212940.15002506758666634No Hit
TTAGGTAGTAGTTTGTGCTGTT209630.14769303521270247No Hit
TGGCTCAGTTCAGCAGGAACAGT206150.14524123078327825No Hit
TAGCACCATCTGAAATCGGTT200000.14090830054162332No Hit
AACCCGTAGATCCGAACTTGTGA198390.13977398872226326No Hit
TAGTAGCACGTAAATATTGGCG195110.13746309259338063No Hit
CAGCAGCAATTCATGTTTTGA194950.13735036595294733No Hit
AAAGGTTCGTTTGTAAAAAAAAA191790.13512401480438968No Hit
TAAAGGTTCGTTTGTAAAA190990.1345603816022232No Hit
AAGGTTCGTTTGTAAAAAAAA189610.133588114328486No Hit
TTCGTGATCGATGTGGTGACG185260.13052335879170568No Hit
TAAAGGTTCGTTTGTAAAAAAAA182360.12848018843385214No Hit
CAACACCAGTCGATGGGCTGTC179460.1264370180759986No Hit
TAGCAGCACGTAAATATTGGCGTT176620.12443612020830756No Hit
ACACGACGCTCTTCCGATCTCAC173870.12249863107586025No Hit
AGGCAAGATGCTGGCATAGCTGT172720.1216884083477459No Hit
TAGGAGCACGTAAATATTGGCG172530.12155454546223136No Hit
ATCCCGAGTGTTGTTCTC170730.12028637075735674No Hit
AGGCAAGATGCTGGCATAGC167870.11827138205961153No Hit
AGCTCGGTCTGAGGCCCCTCAGT164180.11567162391461858No Hit
ATCACATTGCCAGGGATTACCACT161060.11347345442616927No Hit
TCCCTAC160890.11335368237070888No Hit
ATCACATTGCCAGGGATTTC155670.1096759757265725No Hit
CACGACGCTCTTCCGATCTCC151490.10673099224525258No Hit
TAGCTTATCAGACTGATGTTGACT150950.10635053983379021No Hit
GCCCGGATAGCTCAGTCGG149910.10561781667097377No Hit
AAGGTTCGTTTGTAAAAA148570.1046737310573449No Hit
ACCATCGACCGTTGATTGTAC147920.1042157790805846No Hit
TAGCTTATCAGACTGATGTTGACA147870.1041805520054492No Hit
TGAGAACTGAATTCCATGGGTT147600.10399032579971801No Hit
AAAGGTTCGTTTGTAAAA143410.101038296903371No Hit
GTTTCCGTAGTGTAGTGGTTATC142970.10072829864217943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT17250.02660.958326
CGGGCTA21400.02213.024427
GGGCTAA25300.02113.791728
CAGACTA950.01994.051528
CGAGCTA1600.01912.56428
GGACTAA3900.01830.830328
TCCGGGC4350.01794.475825
ACGAGCT1900.01610.580127
ACGACGA1350.01397.37624
CGGACTA5650.01289.549927
CGGCTAA3200.01275.042628
CCCGGGC15700.01238.775325
CGACGGA2250.01117.238625
TCGCCAC1050.01110.241128
CGACGAA600.01102.538125
CACAGGC403.852074E-81092.893728
ACGGACT5850.01071.09826
ACGGTCT4600.01045.376526
TACGGTC1650.01042.399725
CTCATAT850.0970.9707624
Produced by FastQC (version 0.11.5)