FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13895028
Sequences flagged as poor quality0
Sequence length0-34
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG140334410.099612609632741No Hit
TGAGGTAGTAGTTTGTGCTGTT10324557.4303916480053145No Hit
TAGCTTATCAGACTGATGTTGAC4607623.3160206658093814No Hit
TGAGGTAGTAGATTGTATAGTT2996722.1566851106741205No Hit
CTTCGTGATCGATGTGGTGACG2724831.9610108018494097No Hit
TAGCAGCACGTAAATATTGGCGT2292711.6500218639357904No Hit
TTCAAGTAATTCAGGATAGGTT1886171.3574423887450964No Hit
TTCGTGATCGATGTGGTGACG1846821.3291229064094006No Hit
ATCACATTGCCAGGGATTTCC1610631.159141241025207No Hit
CTTCGTGATCGATGTGGTGACGTC1394721.003754724351761No Hit
TGAGGTAGTAGTTTGTACAGTT1204350.8667488831256764No Hit
TGAGGTAGTAAGTTGTATTGTT1169580.8417255438420133No Hit
TTCGTGATCGATGTGGTGACGTC1097200.7896349687096708No Hit
ATCACATTGCCAGGGATTACC1095970.7887497599860899No Hit
TAGCTTATCAGACTGATGTTGA1078740.7763496410370674No Hit
CACAGATGATGAACTTATTG954530.6869579535931846No Hit
GGTTTCGTAAACCGAAGATC913730.6575949325183079No Hit
TCCCTACACGACGCTCTCCCTACACG833940.600171514587808No Hit
TTCAAGTAATCCAGGATAGGCT824380.5932913557281064No Hit
TAGCTTATCAGACTGATGTTG819400.5897073399204377No Hit
TAGCAGCACGTAAATATTGGC813840.5857059086170967No Hit
ACCTCAGAAGGTCTCACTTTT789000.5678290104920983No Hit
AACCCGTAGATCCGATCTTGT709620.5107006621361252No Hit
TAAAGGTTCGTTTGTAAAAA667430.48033728323541336No Hit
AAAGGTTCGTTTGTAAAAAA663740.4776816570646709No Hit
TAAAGGTTCGTTTGTAAAAAA654650.47113974869284175No Hit
AAAGGTTCGTTTGTAAAAAAA630660.45387458017356996No Hit
AGGGGTAAAGCACTGTTACGTAT628940.45263672732433496No Hit
AACCCGTAGATCCGATCTTGTG625230.4499667075158107No Hit
TGAGGTAGTAGGTTGTATAGTT616530.4437054750807267No Hit
TGGCTCAGTTCAGCAGGAAC605000.43540754289951766No Hit
TAAAGGTTCGTTTGTAAAAAAA595730.4287360917876524No Hit
ATCACATTGCCAGGGATTTCCA585960.421704799731242No Hit
TTAGGTAGTAGTTTGTGCTGTT558280.4017840050412277No Hit
AAAGGTTCGTTTGTAAAAA541750.38988766341456815No Hit
CAGCAGCAATTCATGTTTTGA537610.3869081803937351No Hit
ACAGATGATGAACTTATTG509590.36674269386143016No Hit
AAAGGTTCGTTTGTAAAAAAAA471550.3393659948004423No Hit
TAAAGGTTCGTTTGTAAAAAAAA464890.33457291341910217No Hit
AGCTACATTGTCTGCTGGGTTTC453140.32611665122229333No Hit
AACCCGTAGATCCGAACTTGTG450280.32405836101949564No Hit
TTTGGCAATGGTAGAACTCACACT438500.3155805083660141No Hit
AACCGGGCGGAAACACCA423870.30505156232862574No Hit
AAGGTTCGTTTGTAAAAAAA411630.2962426560061628No Hit
TAGCACCATCTGAAATCGGTT394900.28420237800168524No Hit
ACCATCGACCGTTGATTGTACC389440.2802729148872532No Hit
TCGAGGAGCTCACAGTCTAGT384090.2764226167806211No Hit
TGGCTCAGTTCAGCAGGAACA382870.2755446048759312No Hit
TGGCTCAGTTCAGCAGGA347390.250010291451014No Hit
AAGGTTCGTTTGTAAAAAAAA340970.24538993372305545No Hit
ATCACATTGCCAGGGATTTC337660.2430077866701672No Hit
TCCCTACACGACGCTCTCCCTAC333260.2398411863581707No Hit
AAGGTTCGTTTGTAAAAAA330530.23787645480095468No Hit
TTCACAGTGGCTAAGTTCCG328230.23622118645604742No Hit
TTTGGCACTAGCACATTTTTGCT311420.22412333390044265No Hit
AAAGGTTCGTTTGTAAAAAAAAA304980.21948858253470235No Hit
TTCGTGATCGATGTGGTGACGTCG301350.21687613727730523No Hit
TAGGATGGGGTGTGATAGGTGGC297720.21426369201990814No Hit
ACTGGACTTGGAGTCAGAAGGC294890.2122269922737831No Hit
TAAAGGTTCGTTTGTAAAAAAAAA292580.21056452710998494No Hit
TGTAAACATCCTCGACTGGAAGCT289630.2084414655371691No Hit
TCGAGGAGCTCACAGTCTAGTA276310.19885530277448885No Hit
TAAAGGTTCGTTTGTAAAA265890.19135621749016987No Hit
AACCCGTAGATCCGATCTTGTGT260570.18752750984021047No Hit
TGATGTAGTAGTTTGTGCTGTT253650.1825473111677069No Hit
AAGTTTCTCTGAACGTGTAGAGC248300.1786970130610748No Hit
AACCCGTAGATCCGAACTTGT237600.1709964168478106No Hit
AAGGTTCGTTTGTAAAAAAAAA231580.1666639318754881No Hit
TAGCAGCACGTAAATATTGGCGA231030.16626810683648857No Hit
TATTAAAGGTTCGTTTGTAAAAA227830.1639651247914002No Hit
CAGTGCAATGTTAAAAGGGCATT225090.1619931964152933No Hit
AGCTACATTGTCTGCTGGGTTT224470.16154699364405742No Hit
TGGCTCAGTTCAGCAGGAACAGT214420.1543141906587018No Hit
AAAGGTTCGTTTGTAAAAAAAAAA213570.15370246105297522No Hit
AGAGGTAGTAGGTTGCATAGTT213320.15352254058070267No Hit
TGAGGTAGTAGTTTGTGCTGT210200.15127713308674154No Hit
AGCTACATTGTCTGCTGGGTT206190.1483912087114902No Hit
AACATTCAACGCTGTCGGTGAGT204670.1472972922400732No Hit
AACCCGTAGATCCGATCTTGTGA202440.14569240162740227No Hit
TTCACAGTGGCTAAGTTCTGC195240.14051069202595345No Hit
AAGGAGCTCACAGTCTATTGAG193050.13893458868884612No Hit
TATTAAAGGTTCGTTTGTAAAAAA188440.1356168551801407No Hit
TAGGGGTAAAGCACTGTTACGTAT187670.1350627001255413No Hit
TGGCTCAGTTCAGCAGGAACAG184410.13271653716710755No Hit
TAGCTTATCAGACTGATGTTGACA182870.13160822705790876No Hit
TAGGATGGGTGTGATAGGTGGC182200.1311260401922184No Hit
AAGGTTCGTTTGTAAAAAAAAAA181160.13037757102756467No Hit
CAACACCAGTCGATGGGCTGTC181090.13032719329532838No Hit
TCGTACCGTGAGTAATAATGCG177420.12768596076236766No Hit
TATGGCACTGGTAGAATTCACT175140.1260450860552422No Hit
AAGGTTCGTTTGTAAAAA169120.12171260108291973No Hit
AGCAGCATTGTACAGGGCTATGA169090.12169101062624703No Hit
TAGTAGCACGTAAATATTGGCG166800.12004293910023067No Hit
TAGGAGCACGTAAATATTGGCG162090.11665323740261625No Hit
TTAGGTAGTAGATTGTATAGTT161620.1163149869147439No Hit
AGTAAGGTCAGCTAAATAAGCTA161130.11596234278908975No Hit
CTTTCAGTCGGATGTTTGCAGC157810.11357299891731057No Hit
TAGCACCATTTGAAATCAGTGTT157160.113105205689402No Hit
TAAAGTGCTGACAGTGCAGAT156110.11234953970585737No Hit
GGCTGTAGGAATACTTTT152470.10972989762956936No Hit
AACATTCATTGCTGTCGGTGGGTT150930.10862158752037059No Hit
GAAGCGGGTGCTCTTATTTT150320.10818258156802563No Hit
AAGGTTCGTTTGTAAAAAAAAAAA149140.10733335693889928No Hit
TGAGGTAGTAGATTGTATAGT144850.10424592163470273No Hit
CTAGACTGAAGCTCCTTGAGG144050.10367017612343062No Hit
TAGCTTATCAGACTGATGTTGACC143930.10358381429673982No Hit
TATTAAAGGTTCGTTTGTAAAA142620.10264103102203177No Hit
ATATAATACAACCTGCTAAGTG142390.10247550418754105No Hit
GGTATCGTAAACCGAAGATC141950.1021588441563414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)