FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13502900
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG192528614.25831488050715No Hit
TGAGGTAGTAGTTTGTGCTGTT7585955.618015389286746No Hit
TGAGATGAAGCACTGTAGCTC4058523.0056654496441504No Hit
AACCCGTAGATCCGAACTTGTG3023352.239037540083982No Hit
TAGCAGCACGTAAATATTGGCGT2707462.0050952017714714No Hit
TAGCTTATCAGACTGATGTTGAC2243091.6611912996467426No Hit
TTCAAGTAATCCAGGATAGGCT1966831.45659821223589No Hit
TTCAAGTAATTCAGGATAGGTT1860671.3779780639714432No Hit
TGAGGTAGTAGATTGTATAGTT1856251.374704693065934No Hit
TGAGGTAGTAGGTTGTATAGTT1410011.0442275363070155No Hit
AACCCGTAGATCCGATCTTGTG1268180.9391908404861179No Hit
AACCCGTAGATCCGAACTTGT1221530.904642706381592No Hit
TCCCTACACGACGCTCTCCCTACACG1073990.7953772893230343No Hit
CAGCAGCAATTCATGTTTTGA1026520.760221878263188No Hit
AGGGGTAAAGCACTGTTACGTAT1021110.7562153315213769No Hit
TAGCAGCACGTAAATATTGGC1005750.744839997333906No Hit
GGTTTCGTAAACCGAAGATC868320.6430618607854609No Hit
ATCACATTGCCAGGGATTACC785910.5820305267757296No Hit
AACCCGTAGATCCGATCTTGT749460.5550363255300713No Hit
TGAGGTAGTAGTTTGTACAGTT672540.4980707847943775No Hit
TGAGATGAAGCACTGTAGCTCT651030.48214087344200135No Hit
ACCATCGACCGTTGATTGTACC616540.45659821223589003No Hit
TGAGATGAAGCACTGTAGCT575170.4259603492583075No Hit
TCCCTACACGACGCTCTCCCTAC502100.37184604788600967No Hit
ATCACATTGCCAGGGATTTCC485220.35934502958623704No Hit
TGAGGTAGTAAGTTGTATTGTT477060.35330188329914314No Hit
AGCTACATTGTCTGCTGGGTTTC434710.321938250301787No Hit
TAGCAGCACGTAAATATTGGCGA431360.3194573017648061No Hit
TTAGGTAGTAGTTTGTGCTGTT424130.31410289641484423No Hit
ACAGATGATGAACTTATTG419900.31097023602337276No Hit
AACCCGTAGATCCGAACTTGTGT414790.30718586377741075No Hit
TGGCTCAGTTCAGCAGGAACA396400.29356656718186463No Hit
TAGCTTATCAGACTGATGTTGA388780.287923334987299No Hit
AAGCTGCCAGTTGAAGAACTGT367970.27251183079190394No Hit
AACCCGTAGATCCGATCTTGTGA362390.2683793851691118No Hit
CACAGATGATGAACTTATTG355850.26353598115960275No Hit
AACCGGGCGGAAACACCA355310.2631360670670745No Hit
AACCCGTAGATCCGATCTTGTGT352330.26092913374164073No Hit
TAGCTTATCAGACTGATGTTG345230.25567100400654674No Hit
TTCACAGTGGCTAAGTTCTGC331440.2454583830140192No Hit
ACCTCAGAAGGTCTCACTTTT309940.22953587747817136No Hit
TAGGGGTAAAGCACTGTTACGTAT293990.21772360011553077No Hit
TACCCTGTAGAACCGAATTTGT290440.21509453524798378No Hit
TAGGTAGTTTCATGTTGTTGGG279280.2068296440023995No Hit
TCGAGGAGCTCACAGTCTAGTA277480.20549659702730527No Hit
ATCACATTGCCAGGGATTTCCA260720.19308444852587223No Hit
AGCTCGGTCTGAGGCCCCTCAGT249930.18509357249183508No Hit
ACTGGACTTGGAGTCAGAAGGC236050.17481429915055283No Hit
AGCAGCATTGTACAGGGCTATGA231710.17160017477727008No Hit
TAGTAGCACGTAAATATTGGCG231230.17124469558391162No Hit
AACCCGTAGATCCGAACTTGTGA220420.16323900791681786No Hit
TAGGAGCACGTAAATATTGGCG219090.16225403431855379No Hit
TGGCTCAGTTCAGCAGGAAC215660.15971383924934643No Hit
TACCCTGTAGATCCGAATTTGT215540.15962496945100682No Hit
TTCAAGTAATCCAGGATAGGC214840.15910656229402573No Hit
CACCCGTAGAACCGACCTTGCG211050.15629975782979952No Hit
TGGCTCAGTTCAGCAGGA207540.15370031622836575No Hit
AAGGAGCTCACAGTCTATTGAG201050.1488939413014982No Hit
GTTGATGATGATGTTCTGAAA198910.14730909656444172No Hit
TGATGTAGTAGTTTGTGCTGTT194620.14413200127380044No Hit
TTCAAGTAATCCAGGATAGGCTT190070.14076235475342336No Hit
AAGCTGCCAGTTGAAGAACTG183810.1361263136067067No Hit
TTAGATGAAGCACTGTAGCTC178950.13252708677395225No Hit
ATATAATACAACCTGCTAAGTG176140.13044605232949957No Hit
ATCACATTGCCAGGGATTACCACT174730.12940183219900908No Hit
TAGGATGGGGTGTGATAGGTGGC173960.1288315843263299No Hit
TCGAGGAGCTCACAGTCTAGT165380.12247739374504736No Hit
CAGTGGTTTTACCCTATGGTAG162270.12017418480474566No Hit
AACATTCATTGCTGTCGGTGGGTT160420.1188041087470099No Hit
AATCCCGAGTGTTGTTCTC159660.118241266690859No Hit
AACATTCAACGCTGTCGGTGAGT157740.11681934991742515No Hit
GAATCCCGAGTGTTGTTCTC150140.11119092935591615No Hit
CTTCGTGATCGATGTGGTGACG149050.11038369535433129No Hit
TGTAAACATCCCCGACTGGAAGCT148650.11008746269319925No Hit
TGGAAGACTAGTGATTTTGTTGTT146170.10825082019418052No Hit
ATCACATTGCCAGGGATTACCAC146020.10813973294625599No Hit
AGGCAGTGTAGTTAGCTGATTGC140360.10394804079123744No Hit
TGAGATGAAGCACTGTAGCTCA138510.10257796473350168No Hit
GGTATCGTAAACCGAAGATC137560.10187441216331307No Hit
TGGCTCAGTTCAGCAGGAACAG136410.1010227432625584No Hit
TAGCAGCACATCATGGTTTACA135490.10034140814195469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)