FastQCFastQC Report
Wed 27 Apr 2022
EGAF00002441071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9887124
Sequences flagged as poor quality0
Sequence length0-34
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG122767312.416886852030986No Hit
AGGGGTAAAGCACTGTTACGTAT2040852.0641492915432234No Hit
TAGCAGCACGTAAATATTGGCGT1862931.8841980741821382No Hit
GGTTTCGTAAACCGAAGATC1755321.7753595484389595No Hit
TTCAAGTAATCCAGGATAGGCT1705601.7250719218247896No Hit
CAGTGCAATGTTAAAAGGGCATT1553721.5714579891988811No Hit
TTCAAGTAATTCAGGATAGGTT1519721.5370698294064078No Hit
TCCCTACACGACGCTCTCCCTACACG1278901.2935005164292468No Hit
CACAGATGATGAACTTATTG992561.0038915259887506No Hit
TGGAGTGTGACAATGGTGTTT678450.6861955003295195No Hit
TCCCTACACGACGCTCTCCCTAC585300.5919820566627869No Hit
TAGCAGCACGTAAATATTGGC583240.5898985387459488No Hit
ACAGATGATGAACTTATTG580330.5869553168342988No Hit
TAGGGGTAAAGCACTGTTACGTAT525920.5319241470016963No Hit
TAGCTTATCAGACTGATGTTGAC496890.5025627270377109No Hit
TGGAGTGTGACAATGGTGTTTG472350.4777425669992608No Hit
AACCGGGCGGAAACACCA385480.38988081872949104No Hit
ACTGGACTTGGAGTCAGAAGGC368790.37300027793724444No Hit
GGTATCGTAAACCGAAGATC280120.28331798003140246No Hit
AATCCCGAGTGTTGTTCTC268780.27184851732414805No Hit
TGTAACAGCAACTCCATGTGGA248980.25182247132735464No Hit
TAGGTAGTTTCATGTTGTTGGG240320.24306360474491873No Hit
GCCGAAATAGCTCTCCCTACACG240230.24297257726311516No Hit
TTCAAGTAATCCAGGATAGGC231980.2346283914311179No Hit
ACCTCAGAAGGTCTCACTTTT228860.2314727720619262No Hit
GAATCCCGAGTGTTGTTCTC224510.22707311044141854No Hit
ATCACATTGCCAGGGATTACC219210.22171260317965064No Hit
AACCCGTAGATCCGATCTTGT215780.21824344470646873No Hit
AGCAGCATTGTACAGGGCTATGA208480.21086010451573178No Hit
CTGACCTATGAATTGACAGCC208160.21053645124709675No Hit
CAAAGTGCTTACAGTGCAGGTAG204640.20697626529211122No Hit
TGTAACAGCAACTCCATGTGG197410.1996637242538882No Hit
AGCGAACCAACAGCCAAGGGA196310.19855116614295523No Hit
AACCCGTAGATCCGATCTTGTG190300.19247255319140327No Hit
ATCCCGAGTGTTGTTCTC189820.19198707328845072No Hit
TAAAGTGCTGACAGTGCAGAT186010.18813357655876473No Hit
CAAAGTGCTGTTCGTGCAGGTAG179300.1813469720820736No Hit
AAAAGCGTAGTCGATGGACA177560.17958710743387057No Hit
CAGTGCAATGTTAAAAGGGC174960.17695742462621084No Hit
TGTAACAGCAACTCCATGTGGAC170820.17277016046324492No Hit
CTAGCGAACCAACAGCCAAGGGA167870.1697864818930156No Hit
TTGGAATCCCGAGTGTTGTTCTC167450.1693616869779321No Hit
CAGTGCAATGATGAAAGGGCATT167420.16933134448399756No Hit
CAGTGCAATGTTAAAAGGGCATA166530.16843118383060635No Hit
ATGGGTAAAGCACTGTTACGTAT159500.1613209260852802No Hit
TAGCAGCACGTAAATATTGGCGA156440.1582259917039576No Hit
TAAAGTGCTGACAGTGCAGATA156360.15814507838679884No Hit
TAGCGAACCAACAGCCAAGGGA147760.149446896792232No Hit
GGTGTCGTAAACCGAAGATC144370.1460181949776295No Hit
TAGCTTATCAGACTGATGTTGA142290.1439144487315017No Hit
GCGAACCAACAGCCAAGGGA141010.1426198356569615No Hit
TGGAGTGTGACAATGGTGTTTGT139750.14134545091171102No Hit
ACACGACGCTCTTCCGATCTCAC139150.1407386010330203No Hit
CAAAGTGCTTACAGTGCAGGTAGT137880.13945410212312498No Hit
AGCTCGGTCTGAGGCCCCTCAGT134710.13624791193070906No Hit
AAAGCGTAGTCGATGGACA132570.1340834806967122No Hit
AGCAGCATTGTACAGGGCTATG132110.13361822912304933No Hit
TGGAATCCCGAGTGTTGTTCTC131900.13340583166550757No Hit
GCCCGGATAGCTCAGTCGG131790.1332945758544143No Hit
TAGTAGCACGTAAATATTGGCG127320.1287735442581685No Hit
CGAACCAACAGCCAAGGGA124640.12606294813335No Hit
TTCAAGTAATCCAGGATAGGCTT123950.1253650707728557No Hit
TAAAGTGCTTATAGTGCAGGTAG121520.1229073287641583No Hit
TCCCTAC117900.11924600116272437No Hit
TCTAGCGAACCAACAGCCAAGGGA114090.11539250443303836No Hit
CAACGGAATCCCAAAAGCAGCT110900.11216608591133276No Hit
TGTAACAGCAACTCCATGTGGAA109580.11083101617821318No Hit
CTGAAAAGCGTAGTCGATGGACA107660.10888909656640293No Hit
TCCCGAGTGTTGTTCTC107270.10849464414525398No Hit
TTCACAGTGGCTAAGTTCTGC105840.10704831860104112No Hit
AAGCTGCCAGTTGAAGAACTGT105290.10649203954557462No Hit
CCTACACGACGCTCTCCCTACACG105270.10647181121628493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)