FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12261805
Sequences flagged as poor quality0
Sequence length0-34
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG153137212.48896063833995No Hit
AACCCGTAGATCCGAACTTGTG2884502.352426906152887No Hit
GGTTTCGTAAACCGAAGATC2826782.3053539018113565No Hit
TAGCAGCACGTAAATATTGGCGT2265621.8477051298728044No Hit
TTCAAGTAATCCAGGATAGGCT1793361.4625579186751054No Hit
TAGCTTATCAGACTGATGTTGAC1400651.142286963460926No Hit
TCCCTACACGACGCTCTCCCTACACG1299671.0599336720817203No Hit
ATCACATTGCCAGGGATTTCC1289371.0515336037394167No Hit
AACCCGTAGATCCGAACTTGT1253041.021905013168942No Hit
TCCCTACACGACGCTCTCCCTAC1069990.8726203034545077No Hit
TTCAAGTAATTCAGGATAGGTT1060510.8648889784171254No Hit
CACAGATGATGAACTTATTG1039980.8481459295756212No Hit
AACCCGTAGATCCGATCTTGTG881460.7188664311657216No Hit
AACCCGTAGATCCGATCTTGT768750.6269468483636789No Hit
ACAGATGATGAACTTATTG720290.5874257501240641No Hit
AGGGGTAAAGCACTGTTACGTAT662660.5404261444379518No Hit
AACCGGGCGGAAACACCA645350.5263091363791873No Hit
TGAGGTAGTAGGTTGTATAGTT641200.5229246428237931No Hit
TAGCAGCACGTAAATATTGGC624750.5095089996945801No Hit
ATCACATTGCCAGGGATTTCCA565670.4613268601156192No Hit
TGAGGTAGTAGATTGTATAGTT497260.40553572659163967No Hit
ACCTCAGAAGGTCTCACTTTT451800.36846125019929776No Hit
TGAGGTAGTAGTTTGTGCTGTT446350.36401655384341863No Hit
GGTATCGTAAACCGAAGATC442110.3605586616326063No Hit
TGGCTCAGTTCAGCAGGAACA432850.35300675553069066No Hit
AACCCGTAGATCCGATCTTGTGT385870.3146926574023971No Hit
AACCCGTAGATCCGAACTTGTGT362130.29533172318431095No Hit
ATCACATTGCCAGGGATTACC359380.2930889864909775No Hit
CAGCAGCAATTCATGTTTTGA346580.2826500666092798No Hit
AGGCAAGATGCTGGCATAGCTG335050.2732468833095943No Hit
AGCTACATTGTCTGCTGGGTTTC329450.26867985586135157No Hit
GGTGTCGTAAACCGAAGATC296710.24197905610144674No Hit
ACTGGACTTGGAGTCAGAAGGC285660.23296733229732491No Hit
TGAGGTAGTAGTTTGTACAGTT277900.22663873711904567No Hit
TCGAGGAGCTCACAGTCTAGTA273020.22265889891414842No Hit
TGGCTCAGTTCAGCAGGAAC269390.21969848647894827No Hit
TAGCAGCACGTAAATATTGGCGA268370.21886663505087545No Hit
AATCCCGAGTGTTGTTCTC264140.21541689824622068No Hit
GTTTCCGTAGTGTAGTGGTTATC261020.2128724115250569No Hit
GAATCCCGAGTGTTGTTCTC252550.20596478250958974No Hit
AGGCAAGATGCTGGCATAGCT251080.20476593780442603No Hit
TAGCTTATCAGACTGATGTTGA245790.20045172794706814No Hit
TAGCTTATCAGACTGATGTTG245320.20006842385766208No Hit
ATCACATTGCCAGGGATTTC235550.19210059204171No Hit
ACACGACGCTCTTCCGATCTCAC234960.191619423078413No Hit
AACCCGTAGATCCGATCTTGTGA234620.1913421392690554No Hit
TGGCTCAGTTCAGCAGGA230180.18772113893509154No Hit
TCCCTAC225260.183708679105564No Hit
TGTAAACATCCTCGACTGGAAGCT217200.17713542174255748No Hit
TCGAGGAGCTCACAGTCTAGT211110.1721687793925935No Hit
TTCAAGTAATCCAGGATAGGCTT209950.17122275227831465No Hit
CAGTGCAATGTTAAAAGGGCATT206390.1683194276862175No Hit
AGGCAAGATGCTGGCATAGC201760.16454347463525965No Hit
ACCATCGACCGTTGATTGTACC199440.16265142040670194No Hit
TAGGGGTAAAGCACTGTTACGTAT197660.16119975811065337No Hit
TTCACAGTGGCTAAGTTCCG191570.1562331157606894No Hit
TAGTAGCACGTAAATATTGGCG191170.15590689951438635No Hit
AGCTCGGTCTGAGGCCCCTCAGT186660.15222881133731941No Hit
ATCCCGAGTGTTGTTCTC185870.1515845342508709No Hit
TAGGAGCACGTAAATATTGGCG181820.1482815947570525No Hit
GCCCGGATAGCTCAGTCGG178860.1458675945344099No Hit
CACGACGCTCTTCCGATCTCC171390.13977550613470038No Hit
TGTTTCGTAAACCGAAGATC170160.13877239117731852No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC167450.13656227610861532No Hit
TGGCTCAGTTCAGCAGGAACAGT162000.13211757975273625No Hit
AGTAAGGTCAGCTAAATAAGCTA161450.13166903241406955No Hit
TTCAAGTAATCCAGGATAGGC160970.1312775729185059No Hit
AGCAGCATTGTACAGGGCTATGA156050.12726511308897834No Hit
CTCACCTGGAGCATGTTTTCT154940.12635986300548735No Hit
AACCCGTAGATCCGAACTTGTGA147620.12039010569814151No Hit
TGGCTCAGTTCAGCAGGAACAG145420.11859591634347472No Hit
TAGGATGGGGTGTGATAGGTGGC142910.11654890939792306No Hit
AATGACACGATCACTCCCGTTGAGT139940.1141267537691229No Hit
TTGGAATCCCGAGTGTTGTTCTC139830.11403704430138957No Hit
AGGCAAGATGCTGGCATAGCTGT138900.11327859152873497No Hit
AGCTACATTGTCTGCTGGGTTT133580.10893991545290437No Hit
GCCGAAATAGCTCTCCCTACACG131850.10752903018764366No Hit
CTTCGTGATCGATGTGGTGACG130550.10646882738715874No Hit
ATATAATACAACCTGCTAAGTG129660.10574299623913445No Hit
GGCTTGGTCTAGGGGTATGATT127790.10421793528766768No Hit
CTTTCAGTCGGATGTTTGCAGC127500.10398142850909797No Hit
TCCCGAGTGTTGTTCTC125410.10227694862216452No Hit
GGCTCAGTAGCCAGTGTAGATCC123290.10054800251675833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)