FastQCFastQC Report
Wed 27 Apr 2022
EGAF00002441075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21972949
Sequences flagged as poor quality0
Sequence length0-34
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCGTAAACCGAAGATC10093094.5934162046250595No Hit
TCCCTACACGACGCTCTCCCTACACG8486203.862112454727856No Hit
TCCCTACACGACGCTCTCCCTAC6176672.8110336942028127No Hit
AGGGGTAAAGCACTGTTACGTAT4264061.9405952291610926No Hit
AACCGGGCGGAAACACCA3291571.498010121445237No Hit
TAGCAGCACGTAAATATTGGCG2634941.1991744940563054No Hit
GGTATCGTAAACCGAAGATC1447160.6586098206481069No Hit
TAGGGGTAAAGCACTGTTACGTAT1367370.6222969889021269No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC1283250.5840135523001487No Hit
TCCCTAC1018950.463729288226173No Hit
GTTTCCGTAGTGTAGTGGTTATC970700.44177046968069694No Hit
AACCCGTAGATCCGAACTTGTG916180.4169581424869279No Hit
GAATCCCGAGTGTTGTTCTC886760.4035689519872822No Hit
ACACGACGCTCTTCCGATCTCAC880970.4009338937618251No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA867420.39476722036718875No Hit
AATCCCGAGTGTTGTTCTC842520.3834351046825804No Hit
CACGACGCTCTTCCGATCTCC807330.36741995805842903No Hit
GGTGTCGTAAACCGAAGATC779710.35484995664441765No Hit
GCCCGGATAGCTCAGTCGGTAGAGC724570.3297554643211523No Hit
GGCTTGGTCTAGGGGTATGATT712200.32412581488265413No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT653360.29734743388336266No Hit
CCTACACGACGCTCTCCCTACACG604930.2753066964293232No Hit
TACACGACGCTCTTCCGATCTCTAC568880.2589001594642576Illumina Single End PCR Primer 1 (100% over 21bp)
ATCCCGAGTGTTGTTCTC564500.25690679935588073No Hit
TAGCAGCACGTAAATATTGGCGT532150.2421841510668413No Hit
ACCTCAGAAGGTCTCACTTTT505470.2300419483975501No Hit
TTGGAATCCCGAGTGTTGTTCTC500800.22791660782537654No Hit
GCCCGGATAGCTCAGTCGG485620.2210081132031936No Hit
TCCCACATGGTCTAGCGGTTAGGATT468350.2131484490315797No Hit
AACCCGTAGATCCGAACTTGT443480.20182998649839853No Hit
TAGCTTATCAGACTGATGTTGAC402500.18317978164879006No Hit
TACACGACGCTCTTCCGATCCCTAC389860.1774272538474467No Hit
TGGAATCCCGAGTGTTGTTCTC387860.1765170437522974No Hit
GTTGGAATCCCGAGTGTTGTTCTC377540.1718203596613272No Hit
GCCGAAATAGCTCTCCCTACACG372340.16945381341393911No Hit
CCTACACGACGCTCTCCCTAC360360.164001654943995No Hit
TTCAAGTAATTCAGGATAGGTT358990.16337816102881775No Hit
GCATTGGTGGTTCAGTGGTAGAATT348250.1584903328178662No Hit
ACGACGCTCTTCCGATCTC333430.1517456760128101No Hit
GCATGGGTGGTTCAGTGGTAGAATT332030.15110852894620563No Hit
TCCCGAGTGTTGTTCTC324480.14767248583701714No Hit
CGTTGGAATCCCGAGTGTTGTTCTC324260.14757236272655072No Hit
AGCGAACCAACAGCCAAGGGA321220.14618884338192384No Hit
GCCCGGATAGCTCAGTCGGTAGA317510.14450040365542194No Hit
TCCCATATGGTCTAGCGGTTAGGATT311250.14165144605760474No Hit
GTTTCCGTAGTGTAGTGGTCATC298210.13571687623723153No Hit
GCCCGGATAGCTCAGTCGGTAGAG289290.1316573392128658No Hit
GCGAACCAACAGCCAAGGGA280720.12775708895515117No Hit
CTCAGGATGTGAATCTGCGC266380.12123088257293092No Hit
CGAACCAACAGCCAAGGGA259620.1181543724513264No Hit
CCCTACACGACGCTCTCCCTAC259220.11797233043229655No Hit
CTAGCGAACCAACAGCCAAGGGA256930.11693013987335063No Hit
TTCAAGTAATCCAGGATAGGCT256870.11690283357049616No Hit
ATCACATTGCCAGGGATTTCC247830.11278868394042148No Hit
CACAGATGATGAACTTATTG247490.11263394822424609No Hit
TGTTTCGTAAACCGAAGATC244880.1114461240500763No Hit
GGTTCCATAGTGTAGTGGTTATC242090.11017638096734307No Hit
GCCCGGATAGCTCAGTCGGTAG240890.10963025491025351No Hit
TCCCTACACGACGCTCCTCCCTAC240500.10945276394169941No Hit
GTTTCCGTAGTGTAGTGGTTATCAC231510.10536136956400344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)