FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14842730
Sequences flagged as poor quality0
Sequence length0-34
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG192519712.970639498259418No Hit
TGAGGTAGTAGTTTGTGCTGTT5163013.478477342106203No Hit
TGAGGTAGTAGATTGTATAGTT4227612.848269826372911No Hit
TAGCTTATCAGACTGATGTTGAC3808742.566064329136217No Hit
TTCAAGTAATCCAGGATAGGCT2948381.9864135506069303No Hit
TAGCAGCACGTAAATATTGGCGT2917541.9656357017880135No Hit
GGTTTCGTAAACCGAAGATC2389141.609636502179855No Hit
TTCAAGTAATTCAGGATAGGTT2046781.3789781259916472No Hit
TGAGGTAGTAGTTTGTACAGTT1625201.0949468190824734No Hit
TCCCTACACGACGCTCTCCCTACACG1481910.9984079748132587No Hit
TGAGGTAGTAGGTTGTATAGTT1319090.8887111737530764No Hit
AACCGGGCGGAAACACCA1271150.8564125332738654No Hit
CACAGATGATGAACTTATTG1221580.8230157120691408No Hit
TAGCAGCACGTAAATATTGGC1138100.7667726893906984No Hit
TAGCTTATCAGACTGATGTTGA986690.6647631534091101No Hit
ACAGATGATGAACTTATTG925690.6236655925156626No Hit
TCCCTACACGACGCTCTCCCTAC865620.5831946009932135No Hit
TAGCTTATCAGACTGATGTTG829300.558724708998951No Hit
AGGGGTAAAGCACTGTTACGTAT823030.5545004187235097No Hit
ACCTCAGAAGGTCTCACTTTT821840.5536986794208343No Hit
TGAGGTAGTAAGTTGTATTGTT730140.491917591979373No Hit
ATCACATTGCCAGGGATTTCC492390.33173816407089535No Hit
CTGACCTATGAATTGACAGCC472790.3185330461444761No Hit
TACCCTGTAGATCCGAATTTGT450800.3037177123076415No Hit
AACCCGTAGATCCGATCTTGT435680.29353090705011814No Hit
TTCAAGTAATCCAGGATAGGC389330.26230349807616254No Hit
GGTATCGTAAACCGAAGATC381860.2572707311929814No Hit
TAGGTAGTTTCATGTTGTTGGG375070.2526961010541861No Hit
AACCCGTAGATCCGATCTTGTG365580.24630239854797603No Hit
TTCAAGTAATCCAGGATAGGCTT345720.23292211068987984No Hit
TGTAACAGCAACTCCATGTGG320310.2158026185209864No Hit
ACTGGACTTGGAGTCAGAAGGC311000.20953018750593724No Hit
TGTAACAGCAACTCCATGTGGA307770.20735403797010388No Hit
TTAGGTAGTAGTTTGTGCTGTT295580.1991412630964789No Hit
CAGCAGCAATTCATGTTTTGA280990.189311535007374No Hit
TACCCTGTAGAACCGAATTTGT273750.18443372614067627No Hit
ACACGACGCTCTTCCGATCTCAC271300.18278308639987387No Hit
AAAGGTTCGTTTGTAAAAAA270170.18202177092758542No Hit
TAGGATGGGGTGTGATAGGTGGC263860.17777053143188618No Hit
TAGCAGCACGTAAATATTGGCGA251240.1692680524404877No Hit
TTAGGTAGTAGATTGTATAGTT250960.1690794078986817No Hit
AATCCCGAGTGTTGTTCTC246780.16626321438172087No Hit
AAAGGTTCGTTTGTAAAAAAA246520.16608804445004388No Hit
AGAGGTAGTAGGTTGCATAGTT245800.16560295848539994No Hit
TAGGTAGTTTCCTGTTGTTGGG234130.1577405234751289No Hit
TAGGGGTAAAGCACTGTTACGTAT232900.15691183495219546No Hit
GGTGTCGTAAACCGAAGATC231340.15586081536213353No Hit
TCCCTAC228230.15376551348707415No Hit
GAATCCCGAGTGTTGTTCTC225720.15207444991588473No Hit
AAAGGTTCGTTTGTAAAAA225400.15185885615382075No Hit
TAAAGGTTCGTTTGTAAAAA222940.15020147910795387No Hit
CAAAGTGCTGTTCGTGCAGGTAG219970.1482004995037975No Hit
TCGAGGAGCTCACAGTCTAGT219170.14766151509863754No Hit
AACCCGTAGATCCGAACTTGTG218770.14739202289605755No Hit
AGCTCGGTCTGAGGCCCCTCAGT214160.1442861252613232No Hit
TAAAGGTTCGTTTGTAAAAAA211520.14250747672429534No Hit
GTTTCCGTAGTGTAGTGGTTATC209460.14111959188100842No Hit
TAAAGTGCTGACAGTGCAGAT209350.14104548152529892No Hit
TAGTAGCACGTAAATATTGGCG204680.1378991600601776No Hit
GAAGCGGGTGCTCTTATTTT199650.13451029561273434No Hit
GCCCGGATAGCTCAGTCGG192580.1297470209321331No Hit
TAGGAGCACGTAAATATTGGCG191130.12877011169778066No Hit
TAAAGGTTCGTTTGTAAAAAAA184760.12447844837169442No Hit
TGAGGTAGTAGATTGTATAGT183350.12352848835759998No Hit
AAAGGTTCGTTTGTAAAAAAAA180860.12185089939653958No Hit
AAGCTGCCAGTTGAAGAACTGT180010.12127822846605713No Hit
GCCGAAATAGCTCTCCCTACACG177200.11938504574293272No Hit
ATCCCGAGTGTTGTTCTC174600.11763334642616284No Hit
AGCTACATTGTCTGCTGGGTTTC173940.11718868429190588No Hit
TGTAAACATCCTCGACTGGAAGCT172860.11646105534493992No Hit
TAGGATGGGTGTGATAGGTGGC160300.10799900018392843No Hit
TGAGGTAGTAGATTGTATAGTTT154230.10390945600977718No Hit
ACCCTGTAGATCCGAATTTGTG153260.10325593741852071No Hit
CAACACCAGTCGATGGGCTGTC153210.10322225089319823No Hit
TTGGAATCCCGAGTGTTGTTCTC152360.10264957996271576No Hit
TGTAAACATCCCCGACTGGAAGCT151490.10206343442210428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)