FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14996400
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG176828011.791363260515856No Hit
TTCAAGTAATTCAGGATAGGTT6027994.019624709930383No Hit
TAGCAGCACGTAAATATTGGCGT3042472.0288002453922274No Hit
TAGCTTATCAGACTGATGTTGAC2821721.8815982502467257No Hit
TCCCTACACGACGCTCTCCCTACACG2305701.5375023338934677No Hit
TTCAAGTAATCCAGGATAGGCT2166131.4444333306660264No Hit
AACCGGGCGGAAACACCA2135981.4243285055079886No Hit
GGTTTCGTAAACCGAAGATC1670151.1137006214824892No Hit
TAGCAGCACGTAAATATTGGC1439560.9599370515590409No Hit
TGAGGTAGTAGATTGTATAGTT1306170.8709890373689685No Hit
AGGGGTAAAGCACTGTTACGTAT1006650.6712611026646396No Hit
ACAGATGATGAACTTATTG887920.5920887679709796No Hit
CACAGATGATGAACTTATTG881450.5877743991891388No Hit
ACCTCAGAAGGTCTCACTTTT874340.5830332613160492No Hit
ATCACATTGCCAGGGATTACC873590.5825331412872422No Hit
TGAGGTAGTAGGTTGTATAGTT866550.577838681283508No Hit
TGAGGTAGTAGTTTGTACAGTT855320.5703502173855058No Hit
TCCCTACACGACGCTCTCCCTAC843600.5625350084020165No Hit
TAGCTTATCAGACTGATGTTGA671150.4475407431116801No Hit
ATCACATTGCCAGGGATTTCC666260.4442799605238591No Hit
GTTGATGATGATGTTCTGAAA663490.4424328505507989No Hit
TAGCTTATCAGACTGATGTTG584990.3900869542023419No Hit
ACTGGACTTGGAGTCAGAAGGC567780.37861086660798593No Hit
TAGCAGCACGTAAATATTGGCGA567240.37825078018724495No Hit
TGTAACAGCAACTCCATGTGG490110.32681843642474195No Hit
TGTAACAGCAACTCCATGTGGA455570.3037862420314209No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT426940.2846949934650983No Hit
TGAGGTAGTAGTTTGTGCTGTT415730.2772198661011976No Hit
AAGCTGCCAGTTGAAGAACTGT411220.27421247766130535No Hit
AATGACACGATCACTCCCGTTGAGT388160.2588354538422555No Hit
CTGACCTATGAATTGACAGCC376290.25092022085300475No Hit
AGCTACATTGTCTGCTGGGTTTC361080.2407777866688005No Hit
TGGCTCAGTTCAGCAGGAACA335510.22372702782001014No Hit
CAACGGAATCCCAAAAGCAGCT330360.22029287028886932No Hit
TAGGGGTAAAGCACTGTTACGTAT325400.21698540983169293No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA323550.21575178042730256No Hit
TGGCTCAGTTCAGCAGGAAC316180.2108372676108933No Hit
TCGTACCGTGAGTAATAATGCG307770.20522925502120512No Hit
TTCAAGTAATCCAGGATAGGC291360.19428662879090983No Hit
GGTATCGTAAACCGAAGATC281110.187451655063882No Hit
TTTGGCAATGGTAGAACTCACACT279600.18644474673921743No Hit
CAGTGGTTTTACCCTATGGTAG277000.1847109973060201No Hit
AGTAAGGTCAGCTAAATAAGCTA271770.18122349363847323No Hit
CAAAGTGCTTACAGTGCAGGTAG265300.17690912485663227No Hit
TCCCTAC264730.176529033634739No Hit
TTCAAGTAATCCAGGATAGGCTT264430.17632898562321622No Hit
CAAAGTGCTTACAGTGCAGGTAGT263210.17551545704302365No Hit
TCTTTGGTTATCTAGCTGTATG256210.17084767010749247No Hit
GAAAATGTTTAG249340.16626657064362113No Hit
TTCAAGTAATTCAGGATAGGTTT246990.16469952788669281No Hit
CAACGGAATCCCAAAAGCAGCTG244220.1628524179136326No Hit
TGGAGTGTGACAATGGTGTTT240380.1602918033661412No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC239470.15968499106452216No Hit
TGTAAACATCCCCGACTGGAAGCT236730.1578578858926142No Hit
ATATAATACAACCTGCTAAGTG234400.1563041796697874No Hit
TGTAACAGCAACTCCATGTGGAA228210.1521765223653677No Hit
TTCACAGTGGCTAAGTTCTGC224450.14966925395428235No Hit
AGCTCGGTCTGAGGCCCCTCAGT215920.14398122215998507No Hit
ATAAAGCTAGATAACCGAAAGT215280.1435544530687365No Hit
GTTTCCGTAGTGTAGTGGTTATC215070.14341441946067054No Hit
TAGGATGGGGTGTGATAGGTGGC211290.14089381451548372No Hit
GCCCGGATAGCTCAGTCGGTAGAGC209340.13959350244058574No Hit
ATCACATTGCCAGGGATTTCCA208230.1388533247979515No Hit
TAGGGAGGTTATGATTAACTTTT204950.1366661332053026No Hit
AAAGGTTCGTTTGTAAAAAA197370.13161158678082738No Hit
AGCAGCATTGTACAGGGCTATGA197370.13161158678082738No Hit
AAAGGTTCGTTTGTAAAAA196940.13132485129764476No Hit
TGGCTCAGTTCAGCAGGA190400.1269638046464485No Hit
AGCTACATTGTCTGCTGGGTTT187660.12513669947454054No Hit
GAAGCGGGTGCTCTTATTTT186460.1243365074284495No Hit
ATCACATTGCCAGGGATTACCACT185870.12394307967245471No Hit
CAAAGTGCTGTTCGTGCAGGTAG185660.12380304606438879No Hit
GGTGTCGTAAACCGAAGATC182090.12142247472726787No Hit
GAATCCCGAGTGTTGTTCTC181880.12128244111920194No Hit
CCCCCGCGGGGGCGCGCCGGCG180010.12003547518071003No Hit
TCCCATATGGTCTAGCGGTTAGGATT174820.11657464458136618No Hit
TAAAGTGCTTATAGTGCAGGTAG174750.11652796671201089No Hit
GCCCGGATAGCTCAGTCGG173310.11556773625670161No Hit
GGCTTGGTCTAGGGGTATGATT173310.11556773625670161No Hit
AAGGAGCTCACAGTCTATTGAG171960.11466752020484916No Hit
AAAGGTTCGTTTGTAAAAAAA169040.11272038622602758No Hit
TTTGGCACTAGCACATTTTTGCT168700.11249366514630178No Hit
TGTAAACATCCCCGACTGGAAGC167980.11201354991864715No Hit
AAGCTGCCAGTTGAAGAACTG167600.11176015577071831No Hit
AGCAGCATTGTACAGGGCTATGT167510.11170014136726147No Hit
TGAGGTAGTAAGTTGTATTGTT167460.11166680003200768No Hit
TAGCACCATCTGAAATCGGTT166980.11134672321357125No Hit
TAGCAGCACGTAAATATTGG166730.11118001653730228No Hit
TAGCAGCACGTAAATATTGGCA165060.11006641593982555No Hit
TAAAGTGCTGACAGTGCAGAT164600.10975967565549066No Hit
TAGCTTATCAGACTGATGTTGACA164170.10947294017230802No Hit
AATCCCGAGTGTTGTTCTC152890.10195113493905204No Hit
ACTCGGCGTGGCGTCGGTCGTGG150990.10068416419940786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)