FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13901061
Sequences flagged as poor quality0
Sequence length0-34
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG139403510.028263310260995No Hit
TCCCTACACGACGCTCTCCCTACACG3180042.2876239446758775No Hit
TAGCAGCACGTAAATATTGGCGT2945482.1188886229619452No Hit
ACAGATGATGAACTTATTG2779761.9996747010893627No Hit
GGTTTCGTAAACCGAAGATC2387411.7174300580365773No Hit
ATCACATTGCCAGGGATTTCC2086011.5006120755818566No Hit
CACAGATGATGAACTTATTG1952531.4045906280103369No Hit
TTCAAGTAATTCAGGATAGGTT1798191.2935631316199534No Hit
TAGCTTATCAGACTGATGTTGAC1462451.0520419988085803No Hit
TGAGGTAGTAGGTTGTATAGTT1319810.9494311261564855No Hit
TTCAAGTAATCCAGGATAGGCT1264300.9094989224203822No Hit
AGGGGTAAAGCACTGTTACGTAT1107000.7963420921611668No Hit
TCCCTACACGACGCTCTCCCTAC1078940.7761565825802793No Hit
AACCGGGCGGAAACACCA973580.7003638067626636No Hit
TAGCAGCACGTAAATATTGGC936310.6735529036236875No Hit
ATCACATTGCCAGGGATTTCCA883180.6353327994172532No Hit
AACCCGTAGATCCGAACTTGTG829700.5968609158682204No Hit
ACCTCAGAAGGTCTCACTTTT744060.5352541075821479No Hit
TTTGGCAATGGTAGAACTCACACT740120.5324197915540404No Hit
TAGGATGGGGTGTGATAGGTGGC707390.5088748261733402No Hit
TGGCTCAGTTCAGCAGGAAC582660.4191478621667799No Hit
TGGCTCAGTTCAGCAGGAACA532070.3827549566180596No Hit
TAGGATGGGTGTGATAGGTGGC433330.31172440722330474No Hit
TAGCAGCACGTAAATATTGGCGA423100.3043652567239292No Hit
GGTATCGTAAACCGAAGATC413560.2975024712142476No Hit
AGCTACATTGTCTGCTGGGTTTC412100.29645219167083725No Hit
TGGCTCAGTTCAGCAGGA392840.28259713413242343No Hit
TGAGGTAGTAGATTGTATAGTT377700.2717058791411677No Hit
ATCACATTGCCAGGGATTACC365230.26273534084916256No Hit
TAGGGGTAAAGCACTGTTACGTAT356220.25625382120112994No Hit
TAGCTTATCAGACTGATGTTGA355210.255527257955346No Hit
TAGCTTATCAGACTGATGTTG334110.240348560444415No Hit
AACCCGTAGATCCGAACTTGT332440.2391472132954456No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT328970.2366510009559702No Hit
ATCACATTGCCAGGGATTTC321720.23143557171643228No Hit
ATCGTGCATCCCTTTAGAGTGT294900.21214208037789345No Hit
AGTAAGGTCAGCTAAATAAGCTA288890.2078186693807041No Hit
TTTGGCACTAGCACATTTTTGCT278600.20041635670831168No Hit
TCCCTAC276360.19880496891568206No Hit
AAAGTGCTTCCTTTTAGAGGGT261430.18806478152998538No Hit
GGTGTCGTAAACCGAAGATC260930.18770509675484484No Hit
ACTGGACTTGGAGTCAGAAGGC256070.18420896074047874No Hit
TGGCTCAGTTCAGCAGGAACAGT252430.18159045557745557No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC249330.17936040997158417No Hit
TTCAAGTAATCCAGGATAGGCTT248820.17899353150094083No Hit
TAGCACCATCTGAAATCGGTT247070.17773463478794893No Hit
AAGCTGCCAGTTGAAGAACTGT235970.16974963277982882No Hit
TTCACAGTGGCTAAGTTCCG234030.1683540558522835No Hit
AACATTCAACGCTGTCGGTGAGT228920.16467807745034713No Hit
TGAGGTAGTAGTTTGTACAGTT220190.15839798127639323No Hit
GAATCCCGAGTGTTGTTCTC214070.15399543962867293No Hit
TCCCACATGGTCTAGCGGTTAGGATT211740.15231930857651801No Hit
GGCTGTAGGAATACTTTT210410.15136254707464417No Hit
CAAGTGCTTCCTTTTAGAGGGT208380.14990222688757354No Hit
AAGGAGCTCACAGTCTATTGAG204090.14681613151686768No Hit
TGAGGTAGTAGTTTGTGCTGTT203350.14628379804965966No Hit
AATCCCGAGTGTTGTTCTC199020.14316892789694252No Hit
TATGGCACTGGTAGAATTCACT197950.14239920247814178No Hit
CCTACACGACGCTCTCCCTACACG194180.13968717927358207No Hit
TGGCTCAGTTCAGCAGGAACAG194060.13960085492754834No Hit
TCGAGGAGCTCACAGTCTAGT183280.13184605117551818No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA182540.13131371770831016No Hit
AACCCGTAGATCCGAACTTGTGT180610.12992533447626767No Hit
TCGAGGAGCTCACAGTCTAGTA178360.12830675298813524No Hit
TTCAAGTAATCCAGGATAGGC174500.12552998652405023No Hit
CTTCGTGATCGATGTGGTGACG173950.12513433327139561No Hit
TTGGAATCCCGAGTGTTGTTCTC160800.11567462368519928No Hit
TGTAAACATCCTCGACTGGAAGCT157760.11348774025234476No Hit
ATCGTGCATCCTTTTAGAGTGT154930.1114519244250493No Hit
CAAGTCACTAGTGGTTCCGTTT149210.10733713059744145No Hit
AGGCAAGATGCTGGCATAGC148510.10683357191224468No Hit
TGAGGTAGTAGGTTGTATAGT145720.10482653086696045No Hit
CACGACGCTCTTCCGATCTCC145310.1045315893513452No Hit
GTTTCCGTAGTGTAGTGGTTATC142390.10243103026452442No Hit
AGGCAAGATGCTGGCATAGCTG139770.10054628204278797No Hit
CAAAGTGCTGTTCGTGCAGGTAG139040.10002114227108277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA4550.02051.434628
CCGCCAC2250.01935.946728
CGACGGA2400.01660.362325
TCGCCAC1050.01629.750928
CGACGGT3000.01628.226125
ACGGTCT6650.01539.768727
CGGCTAA4550.01504.385428
ACGGACT6500.01366.805927
GACGGTC4900.01352.149926
CCGGGCT6050.01344.022726
CGAGCTA1100.01272.82228
CGGGCTA11100.01261.618927
TTACGTG750.01199.745525
GGGCTAA17100.01173.576728
CCCGCCA3600.01171.180327
ACGACGG11150.01126.607224
ATTACGG1500.01101.900924
TTCGCCA1550.01068.634527
ACGGCTA6350.01043.391127
GCGACTA150.0076058051037.114328
Produced by FastQC (version 0.11.5)