FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12231136
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG122955510.052663955334975No Hit
TAGCTTATCAGACTGATGTTGAC4211623.443359635605393No Hit
AACCCGTAGATCCGAACTTGTG3792933.1010447435136035No Hit
TAGCAGCACGTAAATATTGGCGT2355941.9261824903263276No Hit
CACAGATGATGAACTTATTG2292991.8747154802301276No Hit
GGTTTCGTAAACCGAAGATC1697201.3876061880106638No Hit
ACAGATGATGAACTTATTG1475831.2066172757787992No Hit
TTCAAGTAATCCAGGATAGGCT1455961.1903718509875125No Hit
AACCGGGCGGAAACACCA1409551.1524277058157149No Hit
TAGGATGGGGTGTGATAGGTGGC1372971.1225204265572715No Hit
TGAGGTAGTAGGTTGTATAGTT1232931.008025746749934No Hit
TCCCTACACGACGCTCTCCCTACACG1208630.9881584179915912No Hit
TTCAAGTAATTCAGGATAGGTT1194130.9763034275802347No Hit
AACCCGTAGATCCGAACTTGT1080670.8835401715752323No Hit
TAGGATGGGTGTGATAGGTGGC807070.6598487662961151No Hit
TAGCTTATCAGACTGATGTTGA742130.6067547609641493No Hit
AGCTACATTGTCTGCTGGGTTTC681600.557266307888327No Hit
TGTAAACATCCTCGACTGGAAGCT623650.5098872255201806No Hit
TAGCTTATCAGACTGATGTTG615190.5029704518043132No Hit
TCCCTACACGACGCTCTCCCTAC594990.48645522378297484No Hit
AGGGGTAAAGCACTGTTACGTAT591540.4836345536506176No Hit
TAGCAGCACGTAAATATTGGC565120.46203394353558No Hit
ATCACATTGCCAGGGATTTCC530450.43368825266925326No Hit
ACCTCAGAAGGTCTCACTTTT508670.4158812394858499No Hit
AACCCGTAGATCCGAACTTGTGT484900.396447231066681No Hit
TGAGGTAGTAGTTTGTGCTGTT426880.3490109177103419No Hit
AAAGGTTCGTTTGTAAAAAA415210.33946969439306374No Hit
TAGCTTATCAGACTGATGTTGACC411460.3364037485970232No Hit
AAAGGTTCGTTTGTAAAAA397540.32502295780212076No Hit
AAAGGTTCGTTTGTAAAAAAA373120.3050575187783048No Hit
CTTCGTGATCGATGTGGTGACGTC366900.2999721366846056No Hit
CTTCGTGATCGATGTGGTGACG360260.2945433686617498No Hit
AGCTACATTGTCTGCTGGGTTT338980.2771451482511518No Hit
CTTTCAGTCGGATGTTTGCAGC323280.2643090551850621No Hit
AACCCGTAGATCCGATCTTGTG309600.2531244849211063No Hit
TGAGAACTGAATTCCATGGGTT306880.2509006522370449No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA304930.24930636042310378No Hit
TAAAGGTTCGTTTGTAAAAA299290.24469517794585882No Hit
CAGTGCAATGTTAAAAGGGCATT297730.24341974449470596No Hit
AAAGGTTCGTTTGTAAAAAAAA296980.24280655533549786No Hit
GGTATCGTAAACCGAAGATC294830.24104874641243465No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC287140.23476151356668754No Hit
TGAGGTAGTAGATTGTATAGTT282140.23067358583863345No Hit
ACCATCGACCGTTGATTGTACC278150.22741141951164637No Hit
TTTGGCAATGGTAGAACTCACACT274850.22471338721113068No Hit
TAAAGGTTCGTTTGTAAAAAA266940.2182462855453492No Hit
TAGCACCATCTGAAATCGGTT266810.2181399994244198No Hit
AGGCAAGATGCTGGCATAGCTG264000.21584258404125342No Hit
ATCACATTGCCAGGGATTACC263980.2158262323303412No Hit
GCCCGGATAGCTCAGTCGGTAGAGC257250.21032388160838045No Hit
GGCTCAGTAGCCAGTGTAGATCC253150.20697178087137613No Hit
TAAAGGTTCGTTTGTAAAAAAA240180.19636769634480394No Hit
TCCCTAC233540.19093892832194817No Hit
TAGCAGCACGTAAATATTGGCGA233090.19057101482642333No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT231970.18965531901533922No Hit
ATCACATTGCCAGGGATTTCCA227870.1863032182783349No Hit
AGTAAGGTCAGCTAAATAAGCTA223430.18267313845582292No Hit
TTCGTGATCGATGTGGTGACG219900.17978706147981674No Hit
AGCTCGGTCTGAGGCCCCTCAGT215940.17654942271919793No Hit
TTTGGCACTAGCACATTTTTGCT210300.171938240241953No Hit
AAAGGTTCGTTTGTAAAAAAAAA209260.17108795127451776No Hit
TAGGGGTAAAGCACTGTTACGTAT201860.16503781823699776No Hit
GTTTCCGTAGTGTAGTGGTTATC199200.16286304068567303No Hit
AAGGTTCGTTTGTAAAAAAA197080.1611297593289781No Hit
TAAAGGTTCGTTTGTAAAAAAAA196510.16066373556797997No Hit
TTCGTGATCGATGTGGTGACGTC196020.16026311865063064No Hit
ACTGGACTTGGAGTCAGAAGGC193240.1579902308338326No Hit
TGGCTCAGTTCAGCAGGAACA191640.1566820939608553No Hit
CAACACCAGTCGATGGGCTGTC187600.15337904835658764No Hit
TGAGGTAGTAGTTTGTACAGTT187250.15309289341562388No Hit
AGGCAAGATGCTGGCATAGCT184190.15059108164605478No Hit
AACCCGTAGATCCGAACTTGTGA182330.1490703725312187No Hit
AAGGTTCGTTTGTAAAAAA181590.14846535922746668No Hit
TTCGTGATCGATGTGGTGACGTCG176530.14432837636667598No Hit
TAGCTTATCAGACTGATGTTGACA171020.13982348001036043No Hit
TAGCTTATCAGACTGATGTTGACT170930.13974989731125548No Hit
AACATTCATTGCTGTCGGTGGGTT169990.1389813668983813No Hit
ACCATCGACCGTTGATTGTAC168170.13749336120536965No Hit
AACCCGTAGATCCGATCTTGT167130.1366430722379344No Hit
TGTAAACATCCTCGACTGGAAGC165220.13508148384581775No Hit
TAAAGGTTCGTTTGTAAAA163570.1337324676955599No Hit
AAGGTTCGTTTGTAAAAAAAA162500.13285765116175635No Hit
GGTGTCGTAAACCGAAGATC160790.13145957987876186No Hit
TCCCACATGGTCTAGCGGTTAGGATT159780.13063381847769495No Hit
AAAGGTTCGTTTGTAAAAAAAAAA157380.128671613168229No Hit
GCCCGGATAGCTCAGTCGG149550.12226991834609639No Hit
TTCAAGTAATCCAGGATAGGCTT144220.1179121873879908No Hit
AACCCGTAGATCCGATCTTGTGT143930.11767508757976365No Hit
TAGCACCATTTGAAATCAGTGTT140760.11508334140017738No Hit
AGGCAAGATGCTGGCATAGCTGT139700.11421670072182992No Hit
AGCTACATTGTCTGCTGGGTT139010.11365256669535846No Hit
TAGCACCATCTGAAATCGGTTA138980.11362803912899014No Hit
GCATTGGTGGTTCAGTGGTAGAATT138550.1132764773443775No Hit
GAATCCCGAGTGTTGTTCTC136650.11172306480771697No Hit
AATCCCGAGTGTTGTTCTC135840.11106082051577221No Hit
TCGAGGAGCTCACAGTCTAGTA134240.10975268364279492No Hit
TAAAGGTTCGTTTGTAAAAAAAAA133050.10877975684351807No Hit
AAAGGTTCGTTTGTAAAA130200.10644963803852724No Hit
CAAAGTGCTGTTCGTGCAGGTAG130050.10632700020668563No Hit
TATTAAAGGTTCGTTTGTAAAAA129410.10580374545749471No Hit
AGCAGCATTGTACAGGGCTATGA128800.10530501827467212No Hit
AACATTCAACGCTGTCGGTGAGT128180.10479811523639342No Hit
TTCACAGTGGCTAAGTTCTGC127330.10410316752262423No Hit
TTCAAGTAATCCAGGATAGGC125210.10236988616592932No Hit
GGCTTGGTCTAGGGGTATGATT122460.10012152591549958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)