FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19711828
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG362162418.37284700333221No Hit
TTCAAGTAATTCAGGATAGGTT6033483.060842454591223No Hit
TAGCAGCACGTAAATATTGGCGT4316102.1895990569722907No Hit
TAGCTTATCAGACTGATGTTGAC2645831.342255015618034No Hit
TTCAAGTAATCCAGGATAGGCT2250411.1416546451196714No Hit
CTGACCTATGAATTGACAGCC2138141.0846989939238512No Hit
TCCCTACACGACGCTCTCCCTACACG2095491.0630622385706694No Hit
ACCTCAGAAGGTCTCACTTTT2094801.0627121949318956No Hit
GGTTTCGTAAACCGAAGATC2091261.0609163188721005No Hit
CACAGATGATGAACTTATTG1877780.9526158608932668No Hit
TGTAACAGCAACTCCATGTGGA1443640.7323724618538676No Hit
AATCGTACAGGGTCATCCACTT1294400.6566615739544805No Hit
TAGCAGCACGTAAATATTGGC1229320.6236458637930484No Hit
AACCGGGCGGAAACACCA1223460.6206730294115796No Hit
TGTAACAGCAACTCCATGTGG1124750.5705964966820936No Hit
AGGGGTAAAGCACTGTTACGTAT1051540.5334563592985897No Hit
ACAGATGATGAACTTATTG1002610.5086336995229463No Hit
CAAAGTGCTTACAGTGCAGGTAG988970.5017139962868994No Hit
ACTGGACTTGGAGTCAGAAGGC940340.4770435293976794No Hit
TGTAACAGCAACTCCATGTGGAA826130.4191036975363219No Hit
ATCACATTGCCAGGGATTACC744090.3774840161957582No Hit
TGAGGTAGTAGGTTGTATAGTT715210.3628329143294067No Hit
TTAATATCGGACAACCATTGT654050.33180585788390604No Hit
CAAAGTGCTTACAGTGCAGGTAGT641710.32554565715569356No Hit
TCCCTACACGACGCTCTCCCTAC615080.3120360019375169No Hit
TAGGTAGTTTCCTGTTGTTGGG607730.3083072762201456No Hit
TGAGGTAGTAGATTGTATAGTT585640.29710080668317523No Hit
TAAAGTGCTTATAGTGCAGGTAG512390.2599403769148148No Hit
TGGAAGACTAGTGATTTTGTTGTT494310.25076821895970275No Hit
TGAGGTAGTAGTTTGTACAGTT479030.2430165279445417No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT472310.23960740728865937No Hit
TAGCTTATCAGACTGATGTTGA453370.22999896305913384No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA450900.22874590829424854No Hit
GGCTGTAGGAATACTTTT438910.22266326593352986No Hit
ATCATAGAGGAAAATCCACGT435390.22087753606616292No Hit
TAGCAGCACGTAAATATTGGCGA431760.21903600214044075No Hit
TAGCTTATCAGACTGATGTTG417040.2115684045132699No Hit
TGTCTTGCAGGCCGTCATGC385050.19533956972432998No Hit
CTTCGTGATCGATGTGGTGACG380450.19300594546583907No Hit
AATGACACGATCACTCCCGTTGAGT377710.19161591710317277No Hit
AACCCGTAGATCCGATCTTGTG375200.19034256995343102No Hit
AGCAGCATTGTACAGGGCTATGA372070.1887546908384144No Hit
TAGGGGTAAAGCACTGTTACGTAT372010.1887242522611297No Hit
GCCCGGATAGCTCAGTCGGTAGAGC368660.18702476502940266No Hit
TTGTACATGGTAGGCTTTCATT364980.18515786562260994No Hit
CAAAGTGCTGTTCGTGCAGGTAG352930.17904478468460663No Hit
TGTCTTGCAGGCCGTCATGCA330310.16756944104828836No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC324620.1646828493024594No Hit
TTCGTGATCGATGTGGTGACG324270.16450529093496555No Hit
GGTATCGTAAACCGAAGATC322900.16381027675363238No Hit
ACCTCAGAAGGTCTCACTTT318460.16155782203456726No Hit
TAGGTAGTTTCATGTTGTTGGG315650.16013228199840218No Hit
AATCATACACGGTTGACCTATT312380.15847337953638801No Hit
GTTGATGATGATGTTCTGAAA308750.15663184561066584No Hit
TCCCTAC306370.15542444871170752No Hit
TCGTACCGTGAGTAATAATGCG304790.15462289950987804No Hit
TAGTAGACCGTATAGCGTACG301870.1531415554153577No Hit
TAAAGTGCTTATAGTGCAGGTA288340.14627765623766603No Hit
GCCCGGATAGCTCAGTCGG285820.14499923599171016No Hit
AACCCGTAGATCCGATCTTGT285640.14490792025985616No Hit
TATACAAGGGCAAGCTCTCTGT285430.14480138523935984No Hit
CAGTGGTTTTACCCTATGGTAG284310.14423319846337945No Hit
TAGGTAGTTTCCTGTTGTTGGGA275350.13968770425553634No Hit
AGCTCGGTCTGAGGCCCCTCAGT272600.1382926027966559No Hit
ATATAATACAACCTGCTAAGTG269320.1366286272384276No Hit
CTGCAGTGAAGGCACTTGTAGC266080.13498494406505576No Hit
AGCTACATTGTCTGCTGGGTTTC262150.1329912172529103No Hit
TAAAGTGCTGACAGTGCAGATA259270.13153016554324642No Hit
AACATAGAGGAAATTCCACGT249090.12636575359728178No Hit
TAAGGTGCATCTAGTGCAGATAG247510.12556420439545232No Hit
ATAGTAGACCGTATAGCGTACG242550.12304794867325343No Hit
TTTGGCAATGGTAGAACTCACACT239840.12167313959922944No Hit
TGTAAACATCCCCGACTGGAAGCT237490.12048096198891346No Hit
TCGGATCCGTCTGAGCTTGGCT232670.11803572961371213No Hit
AATCCCGAGTGTTGTTCTC232300.11784802505379002No Hit
GCCCGGATAGCTCAGTCGGTAGA232070.1177313438408655No Hit
TCGTACAGGGTCATCCACTTT230970.11717330325731333No Hit
TTCAAGTAATCCAGGATAGGC230820.11709720681410167No Hit
AAGCTGCCAGTTGAAGAACTGT226940.11512884548302672No Hit
TTCAAGTAATCCAGGATAGGCTT226920.11511869929059851No Hit
TAAAGTGCTGACAGTGCAGAT223170.11321628821030702No Hit
TAGGGAGGTTATGATTAACTTTT221200.11221688825612723No Hit
TTCACAGTGGCTAAGTTCTGC218550.11087251775938792No Hit
TTTGGCACTAGCACATTTTTGCT210740.10691042961616751No Hit
TCGAGGAGCTCACAGTCTAGTA206900.10496236066994903No Hit
TATGTCTGCTGACCATCACC206080.10454636678039195No Hit
GCCCGGATAGCTCAGTCGGTAG203860.1034201394208594No Hit
ATCACATTGCCAGGGATTTCC200440.10168514051563356No Hit
GCCGAAATAGCTCTCCCTACACG197820.10035598930753656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)