FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15214654
Sequences flagged as poor quality0
Sequence length0-34
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG181140311.905647016356731No Hit
TGAGGTAGTAGTTTGTGCTGTT7281244.785675704488581No Hit
TGAGGTAGTAGATTGTATAGTT4896863.2185155179999496No Hit
TAGCTTATCAGACTGATGTTGAC4305852.8300676439963737No Hit
AACCCGTAGATCCGAACTTGTG2806681.8447215427968326No Hit
TAGCAGCACGTAAATATTGGCGT2735961.798240038846759No Hit
TGAGGTAGTAGTTTGTACAGTT2474261.626234812832418No Hit
TTCAAGTAATTCAGGATAGGTT1952911.2835717460285327No Hit
TGAGGTAGTAGGTTGTATAGTT1688141.1095487284824224No Hit
CACAGATGATGAACTTATTG1420900.9339022760557026No Hit
AGCTACATTGTCTGCTGGGTTTC1283160.8433711341710433No Hit
TTCAAGTAATCCAGGATAGGCT1278600.8403740236222264No Hit
GGTTTCGTAAACCGAAGATC1199480.7883715265559113No Hit
ACCTCAGAAGGTCTCACTTTT1127170.7408449774802635No Hit
ATCACATTGCCAGGGATTTCC1082630.7115705687424768No Hit
TCCCTACACGACGCTCTCCCTACACG961760.6321274213662696No Hit
AGGGGTAAAGCACTGTTACGTAT944700.6209145472516168No Hit
ATCACATTGCCAGGGATTACC938480.6168263833012568No Hit
AACCCGTAGATCCGAACTTGT928700.6103983698873467No Hit
AACCGGGCGGAAACACCA882970.5803418204580926No Hit
ACAGATGATGAACTTATTG756990.49754006893617164No Hit
TAGCAGCACGTAAATATTGGC698830.4593137642170502No Hit
TTAGGTAGTAGTTTGTGCTGTT652280.42871826069787716No Hit
ATCACATTGCCAGGGATTACCACT589770.3876328702578448No Hit
ATCACATTGCCAGGGATTTCCA565230.37150368322539573No Hit
AGGCAAGATGCTGGCATAGCTG563140.37013000755718795No Hit
TAGCTTATCAGACTGATGTTGA561130.3688089127758015No Hit
ACTGGACTTGGAGTCAGAAGGC554990.36477332971226295No Hit
GGCTCAGTAGCCAGTGTAGATCC525910.3456601773527022No Hit
TAGCTTATCAGACTGATGTTG513710.3376415921124463No Hit
TCCCTACACGACGCTCTCCCTAC478280.314354831861441No Hit
TGCCGTGATCGTATAGTGGTTAGT472980.3108713481095265No Hit
TTAGGTAGTAGATTGTATAGTT458030.3010452948847867No Hit
GAAGCGGGTGCTCTTATTTT438140.28797237189882857No Hit
TCGAGGAGCTCACAGTCTAGTA436200.28669728539341083No Hit
TTCACAGTGGCTAAGTTCTGC428880.2818861342492573No Hit
TTTGGCAATGGTAGAACTCACACT428770.28181383552987793No Hit
CAGTGCAATGTTAAAAGGGCATT420300.27624683413766754No Hit
AGAGGTAGTAGGTTGCATAGTT417310.27428162349271956No Hit
TGAGGTAGTAAGTTGTATTGTT417230.27422904260589825No Hit
TTTGGCACTAGCACATTTTTGCT393700.25876368926956866No Hit
AACCCGTAGATCCGAACTTGTGT378260.24861557811304813No Hit
AGGCAAGATGCTGGCATAGCT376950.24775456609134852No Hit
TGTAAACATCCTCGACTGGAAGCT350860.23060662437673574No Hit
TGTAAACATCCCCGACTGGAAGCT330050.2169290211923321No Hit
AGCTACATTGTCTGCTGGGTTT328640.21600228306210578No Hit
AATGACACGATCACTCCCGTTGAGT322870.21220988660011592No Hit
CAAAGTGCTTACAGTGCAGGTAGT322080.21169065034275508No Hit
TGGCTCAGTTCAGCAGGAACAGT319160.20977144797377584No Hit
TAGGGGTAAAGCACTGTTACGTAT312930.2056767114125632No Hit
CAAAGTGCTTACAGTGCAGGTAG311300.20460537584357816No Hit
TGGCTCAGTTCAGCAGGAACA309540.20344859633350848No Hit
AAGCTGCCAGTTGAAGAACTGT306700.20158197485135054No Hit
AGGCAAGATGCTGGCATAGCTGT301360.19807220065602543No Hit
TCGTACCGTGAGTAATAATGCG298120.19594267473976076No Hit
AGGCAAGATGCTGGCATAGC294830.19378028576923276No Hit
TAGCTTATCAGACTGATGTTGACC293510.19291270113668046No Hit
TAGCACCATCTGAAATCGGTT279630.18378991727317623No Hit
TCCCTAC272300.17897219351817006No Hit
TCGAGGAGCTCACAGTCTAGT260690.17134139231822162No Hit
TGGCTCAGTTCAGCAGGA254010.1669508882686389No Hit
TGGCTCAGTTCAGCAGGAAC253170.16639878895701474No Hit
GGCTGTAGGAATACTTTT251380.16522229161438703No Hit
AGCTCGGTCTGAGGCCCCTCAGT242950.15968158066558727No Hit
TTAGGTAGTAGTTTGTACAGTT233110.15321413158656122No Hit
ATCACATTGCCAGGGATTACCAC231910.15242541828424097No Hit
TAGCAGCACGTAAATATTGGCGA229370.15075597512766312No Hit
GAAGCGGGTGCTCTTATTT228730.1503353280330923No Hit
TAGCTTATCAGACTGATGTTGACA228460.15015786754007027No Hit
AGCAGCATTGTACAGGGCTATGA209410.13763704386573627No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC207870.1366248617944253No Hit
TAGCACCATTTGAAATCAGTGTT207010.13605961726109578No Hit
TAGCACCATCTGAAATCGGTTA205540.13509344346575347No Hit
AATCCCGAGTGTTGTTCTC202870.13333855636809092No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA199470.13110386867818355No Hit
TAAAGTGCTTATAGTGCAGGTAG195500.12849454216967404No Hit
TTCAAGTAATCCAGGATAGGCTT195220.12831050906579933No Hit
TGAGGTAGTAGATTGTATAGTTT190970.1255171494534151No Hit
TGTAAACATCCCCGACTGGAAGC184700.1213961224487918No Hit
GGTATCGTAAACCGAAGATC182920.12022619771701677No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT181750.11945720224725452No Hit
CTAGACTGAAGCTCCTTGAGG180930.11891824815733569No Hit
TGGCTCAGTTCAGCAGGAACAG180460.11860933544726025No Hit
TAGTGGGTTATCAGAACTT172510.1133841098193886No Hit
CAAAGTGCTGTTCGTGCAGGTAG169060.11111655907521789No Hit
ATATAATACAACCTGCTAAGTG165480.10876356438996246No Hit
CAACGGAATCCCAAAAGCAGCT161940.10643686014811773No Hit
AAGCTGCCAGTTGAAGAACTG161560.1061871009357163No Hit
GTTTCCGTAGTGTAGTGGTTATC160420.10543782329851206No Hit
CAACGGAATCCCAAAAGCAGCTG160060.105201209307816No Hit
ACCTCAGAAGGTCTCACTTT157670.10363035531402817No Hit
TTAGGTAGTAGGTTGTATAGTT156740.10301910250472997No Hit
AGCAGCATTGTACAGGGCTATGT156470.10284164201170792No Hit
GAATCCCGAGTGTTGTTCTC154580.10159941856055354No Hit
AAGGAGCTCACAGTCTATTGAG153500.10088957658846531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)