FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16053006
Sequences flagged as poor quality0
Sequence length0-34
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG13482938.399006391700096No Hit
TCCCTACACGACGCTCTCCCTACACG5741893.576831653834802No Hit
TTCAAGTAATTCAGGATAGGTT4688902.9208859698924927No Hit
TTCAAGTAATCCAGGATAGGCT3869572.410495579457206No Hit
CACAGATGATGAACTTATTG2234471.3919324517788132No Hit
GGTTTCGTAAACCGAAGATC2114431.3171551795345993No Hit
TAGCAGCACGTAAATATTGGCGT1620111.0092253126922146No Hit
ACAGATGATGAACTTATTG1581800.985360623424672No Hit
TAGCTTATCAGACTGATGTTGAC1579870.9841583563850907No Hit
CAGTGCAATGTTAAAAGGGCATT1546090.9631155685109691No Hit
AGGGGTAAAGCACTGTTACGTAT1471080.9163891174026846No Hit
ATCACATTGCCAGGGATTACC1252100.7799785286319585No Hit
TCCCTACACGACGCTCTCCCTAC1080790.6732633128025991No Hit
TACCCTGTAGAACCGAATTTGT905880.5643055263294613No Hit
ACCTCAGAAGGTCTCACTTTT880860.5487196603552008No Hit
TTTGGCAATGGTAGAACTCACACT834950.5201206552841257No Hit
ACTGGACTTGGAGTCAGAAGGC776010.4834047903551522No Hit
CTGACCTATGAATTGACAGCC680070.4236402826984554No Hit
TGGAGTGTGACAATGGTGTTT645510.40211160451818184No Hit
TAGGTAGTTTCATGTTGTTGGG571290.35587727307894856No Hit
TAGCAGCACGTAAATATTGGC562020.35010265367121896No Hit
CAAAGTGCTTACAGTGCAGGTAG556710.34679486197164566No Hit
TGTAACAGCAACTCCATGTGGA516790.321927245277302No Hit
CAAAGTGCTTACAGTGCAGGTAGT507400.31607787351477973No Hit
TGTAACAGCAACTCCATGTGG502430.31298188015378553No Hit
TAGGGGTAAAGCACTGTTACGTAT498550.31056488734882426No Hit
AGCTCGGTCTGAGGCCCCTCAGT490840.30576204855339867No Hit
TTCAAGTAATCCAGGATAGGC484680.3019247610073777No Hit
TAGGTAGTTTCCTGTTGTTGGG479940.29897204299306934No Hit
TGAGGTAGTAGATTGTATAGTT476230.2966609493573976No Hit
ATATAATACAACCTGCTAAGTG438300.27303297588003145No Hit
CAGTGCAATGTTAAAAGGGCATA434380.2705910656234726No Hit
TTTGGCACTAGCACATTTTTGCT434290.2705350013573782No Hit
AACCCGTAGATCCGATCTTGTG429750.2677068706010575No Hit
CAGTGCAATGATGAAAGGGCATT418540.26072375479084725No Hit
TCCCTAC405700.2527252528280373No Hit
AAGTTTCTCTGAACGTGTAGAGC401220.24993449824911299No Hit
AACCCGTAGATCCGATCTTGT398320.24812798300829142No Hit
TAGGATGGGGTGTGATAGGTGGC389190.24244057468115315No Hit
AACCGGGCGGAAACACCA375790.23409322839597768No Hit
TAAAGTGCTTATAGTGCAGGTAG361210.22501081728867478No Hit
GCCGAAATAGCTCTCCCTACACG349550.2177473801479922No Hit
CCTACACGACGCTCTCCCTACACG346360.21576021338308848No Hit
CAAAGTGCTGTTCGTGCAGGTAG345780.21539891033492417No Hit
TAAAGTGCTGACAGTGCAGATA340190.21191669647416816No Hit
TAAAGTGCTGACAGTGCAGAT337480.2102285391284349No Hit
TTCAAGTAATCCAGGATAGGCTT321040.19998746652184646No Hit
TGGCTCAGTTCAGCAGGAACA314780.1960878853468316No Hit
CAGTGGTTTTACCCTATGGTAG314430.19586985764535317No Hit
GGTATCGTAAACCGAAGATC306530.19094866095483923No Hit
TGGAGTGTGACAATGGTGTTTG303820.18926050360910598No Hit
GGCTGTAGGAATACTTTT299990.18687465761864164No Hit
TAGCTTATCAGACTGATGTTGA295440.1840402974994216No Hit
TAGCTTATCAGACTGATGTTG292720.18234591079078896No Hit
TATGGCACTGGTAGAATTCACT289860.18056431300156495No Hit
CAGTGCAATGTTAAAAGGGC281550.17538771243217627No Hit
ATCACATTGCCAGGGATTTCC275740.17176845258763374No Hit
CCCCCGCGGGGGCGCGCCGGCG275210.17143829635396637No Hit
TAGGTAGTTTCCTGTTGTTGGGA268070.16699053124380567No Hit
TAAGGTGCATCTAGTGCAGATAG261180.1626985002061296No Hit
AAGTTTCTCTGAACGTGTAGAGA257500.16040609465915603No Hit
TAGCAGCACGTAAATATTGGCGA243240.15152302316463348No Hit
TGTAACAGCAACTCCATGTGGAC237740.1480968735699719No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA231340.14411008131436567No Hit
TAGGATGGGTGTGATAGGTGGC227550.14174915277549888No Hit
TCAGTGCACTACAGAACTTTGT225810.14066524363100594No Hit
CATTGCACTTGTCTCGGTCTGA224630.13993017880887854No Hit
TGGCTCAGTTCAGCAGGAAC219560.1367718918188905No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAT217100.13523946854564187No Hit
CAAAGAATTCTCCTTTTGGGCT216990.13517094555374862No Hit
TAGGGAGGTTATGACTAACTTTT215430.1341991649414446No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC214330.1335139350225123No Hit
TGTAACAGCAACTCCATGTGGAA211400.13168873169299258No Hit
TGTTTCGTAAACCGAAGATC208240.129720253016787No Hit
AGCAGCATTGTACAGGGCTATGA207170.12905371118655284No Hit
TCGAGGAGCTCACAGTCTAGTA207150.1290412524607541No Hit
AATCCCGAGTGTTGTTCTC202980.12644360813171066No Hit
TTCAAGTAATCCAGGATAGG198370.12357187183509433No Hit
CTTCGTGATCGATGTGGTGACG197630.12311089898053985No Hit
TGTAAACATCCCCGACTGGAAGCT194960.12144765908640412No Hit
TCGAGGAGCTCACAGTCTAGT188090.11716808677452685No Hit
TGAGGTAGTAGGTTGTATAGTT184490.11492551613074835No Hit
CAGTGCAATGATGAAAGGGCATA184370.11485076377595574No Hit
CAGTGCCTCGGCAGTGCAGCC184310.11481338759855941No Hit
AAGGAGCTCACAGTCTATTGAG183460.11428389175211172No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT177550.11060233827857537No Hit
GGTGTCGTAAACCGAAGATC174460.1086774651426655No Hit
TTCACAGTGGCTAAGTTCTG172980.10775551943355656No Hit
TCCCATATGGTCTAGCGGTTAGGATT170430.10616703189421346No Hit
GTTTCCGTAGTGTAGTGGTTATC170110.10596769228143316No Hit
TGGCTCAGTTCAGCAGGA163950.10213040473541217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA10000.02091.800528
CGACGGA7400.01852.893225
ACGGACT11050.01793.346327
CGACGGT4900.01730.213725
TCGCCAC2300.01579.33428
CCGCCAC2800.01520.984128
ACGGTCT8750.01514.458127
GACGGCC2400.01469.012826
CGGCCTA3100.01414.197928
CGAGCTA1100.01366.445828
GACGGTC7700.01357.833726
ACGACGG19100.01350.713524
GACGGAC12800.01272.722426
CGACGTA750.01256.00725
TCAGACA2750.01193.630527
CAGACTA1400.01163.105628
CCCGCCA3600.01148.19427
ACGACGT2500.01114.644724
TTCGCCA3150.01080.653227
CGTGCTA4100.01069.271528
Produced by FastQC (version 0.11.5)