FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002441085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12949106
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG260376520.10768156504395No Hit
TAGCAGCACGTAAATATTGGCGT3735782.88497136404629No Hit
TAGCTTATCAGACTGATGTTGAC3425192.645116967920411No Hit
TTCAAGTAATCCAGGATAGGCT1800191.3902040804979123No Hit
TTCAAGTAATTCAGGATAGGTT1689731.3049008943165652No Hit
ATCACATTGCCAGGGATTTCC1312651.01369932410778No Hit
TCCCTACACGACGCTCTCCCTACACG1285820.992979747018829No Hit
TAGCAGCACGTAAATATTGGC1207400.9324195817070305No Hit
TGAGGTAGTAGATTGTATAGTT1098080.8479967651820906No Hit
AACCCGTAGATCCGATCTTGTG1063140.8210142074672954No Hit
GGTTTCGTAAACCGAAGATC989250.7639523531585887No Hit
TAGCTTATCAGACTGATGTTGA860690.6646713680465663No Hit
AACCCGTAGATCCGAACTTGTG799590.6174866434794803No Hit
AACCCGTAGATCCGATCTTGT775070.5985509733258806No Hit
TAGCTTATCAGACTGATGTTG768130.5931915299789808No Hit
ACAGATGATGAACTTATTG764940.5907280394492098No Hit
AGGGGTAAAGCACTGTTACGTAT745040.5753601831663128No Hit
TAGCAGCACGTAAATATTGGCGA700120.540670529687532No Hit
CACAGATGATGAACTTATTG698190.5391800793043164No Hit
ATCACATTGCCAGGGATTACC693170.5353033637997866No Hit
ATCGTGCATCCCTTTAGAGTGT691930.5343457687349227No Hit
AAAGTGCTTCCTTTTAGAGGGT666030.5143443879446195No Hit
TGAGGTAGTAGTTTGTACAGTT656990.5073632110201276No Hit
AACCGGGCGGAAACACCA614070.47421806571048225No Hit
TGAGGTAGTAGTTTGTGCTGTT610830.47171596247648295No Hit
ATCACATTGCCAGGGATTTCCA606410.4683025994226937No Hit
TGAGGTAGTAGGTTGTATAGTT587660.45382283533704953No Hit
TTCACAGTGGCTAAGTTCCG560630.43294880743118486No Hit
CAAGTGCTTCCTTTTAGAGGGT522300.4033483083697052No Hit
TGGCTCAGTTCAGCAGGAACA517770.39984999736661353No Hit
ACCTCAGAAGGTCTCACTTTT505440.39032810450389394No Hit
ATCGTGCATCCTTTTAGAGTGT463460.3579088780337423No Hit
TCCCTACACGACGCTCTCCCTAC419840.32422315486489955No Hit
ATCACATTGCCAGGGATTTC384880.2972251520684131No Hit
AACCCGTAGATCCGATCTTGTGA359130.277339609390795No Hit
AACCCGTAGATCCGAACTTGT349910.2702194267310809No Hit
TGGCTCAGTTCAGCAGGAAC316670.24454970096005083No Hit
AACCCGTAGATCCGATCTTGTGT303450.23434050196206596No Hit
TGGCTCAGTTCAGCAGGA266600.20588293894574652No Hit
CTTCGTGATCGATGTGGTGACG263910.20380557545825942No Hit
AACATTCAACGCTGTCGGTGAGT244720.18898601957540545No Hit
TGTAAACATCCTCGACTGGAAGCT244360.18870800810496108No Hit
TTCAAGTAATCCAGGATAGGC242790.1874955691921898No Hit
ACTGGACTTGGAGTCAGAAGGC237480.18339490000313535No Hit
TAGGGGTAAAGCACTGTTACGTAT235990.1822442414171295No Hit
GAAAGCGCTTCTCTTTAGAGGA225200.17391161984464412No Hit
TGGCTCAGTTCAGCAGGAACAGT223620.17269145839102715No Hit
TGTAAACATCCCCGACTGGAAGCT217100.1676563617596458No Hit
AGCTCGGTCTGAGGCCCCTCAGT214750.16584156466091174No Hit
TCCCTAC213490.16486852451435643No Hit
GAAAATGTTTAG209480.16177178563523997No Hit
TAGCACCATCTGAAATCGGTT201620.15570186853053794No Hit
CAAAGTGCTTACAGTGCAGGTAG194370.15010302641742218No Hit
TGTAAACATCCCCGACTGGAAGC193910.14974778953852103No Hit
TAGCAGCACATCATGGTTTACA193090.14911454118917553No Hit
TTTGGCAATGGTAGAACTCACACT191230.1476781485918796No Hit
AGTAAGGTCAGCTAAATAAGCTA190360.14700628753830575No Hit
AAGCTGCCAGTTGAAGAACTGT179730.13879722661935118No Hit
ACCATCGACCGTTGATTGTACC178280.13767745819672803No Hit
TAGCTTATCAGACTGATGTTGACA177730.13725271845021578No Hit
TGGCAGCACGTAAATATTGGCG177180.13682797870370356No Hit
TTCAAGTAATCCAGGATAGGCTT177090.13675847583609246No Hit
TGGCTCAGTTCAGCAGGAACAG174570.13481239554298188No Hit
AAAGTGCATCCTTTTAGAGTGT174230.13454982915422886No Hit
CAAAGTGCTGTTCGTGCAGGTAG173860.13426409514293883No Hit
TACCAGCACGTAAATATTGGCG172830.1334686734358341No Hit
CTTCGTGATCGATGTGGTGACGTC171430.13238751771743934No Hit
TGAGGTAGTAAGTTGTATTGTT160040.12359154369421332No Hit
CAAAGCGCTTCTCTTTAGAGTG156510.12086548677568938No Hit
TAAAGTGCTGACAGTGCAGATA156010.12047935973340552No Hit
GGTATCGTAAACCGAAGATC151160.11673392742325223No Hit
TAGCGGCACGTAAATATTGGCG150580.11628602005420298No Hit
CAAAGTGCTTACAGTGCAGGTAGT149170.11519714179496253No Hit
TAGCAGCACGTAAATATTGG147730.11408509591318504No Hit
AGCAGCATTGTACAGGGCTATGA144770.11179922382286468No Hit
TAGGATGGGGTGTGATAGGTGGC143900.11112736276929079No Hit
AATCCCGAGTGTTGTTCTC143020.11044777917487121No Hit
TTCGTGATCGATGTGGTGACG141890.10957513205930973No Hit
AGCTACATTGTCTGCTGGGTTTC141410.10920445009871725No Hit
CACCCGTAGAACCGACCTTGCG141330.10914266977195182No Hit
GCCGAAATAGCTCTCCCTACACG140870.10878743289305069No Hit
GGCTGTAGGAATACTTTT138700.1071116415295388No Hit
GAATCCCGAGTGTTGTTCTC137120.10589148007592185No Hit
TAGCAGCACGTAAATATTGGCA136200.10518100631811958No Hit
TTCACAGTGGCTAAGTTCTGC135170.10438558461101485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT18200.02383.140426
CGGGCTA21550.02183.44827
GGGCTAA25900.02048.5328
TCCGGGC4850.01989.692525
CGACGGA2500.01810.865825
CGGACTA4250.01747.122328
CCGCCAC950.01705.32528
GGACTAA1700.01508.878428
ACGGACT5250.01401.960727
TCGCCTA750.01260.045728
TACCCGG1350.01243.726623
CGAGCTA900.01200.043628
CTACCCG1100.01198.035422
AACGGAC1250.01156.617426
CTAAAAT600.01125.040928
ACGACGG6700.01084.231824
ACGGTCT2200.01015.624927
GACGGAC5250.0976.365426
CGCCAAC1000.0945.0343628
ATTACGG800.0934.7514624
Produced by FastQC (version 0.11.5)