FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002441086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13086260
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG214328316.378117200789223No Hit
TAGCTTATCAGACTGATGTTGAC4162313.1806719414103033No Hit
TAGCAGCACGTAAATATTGGCGT3978833.0404638147186436No Hit
AACCGGGCGGAAACACCA3036952.3207165378037726No Hit
GGTTTCGTAAACCGAAGATC2111321.6133868653075822No Hit
TGAGGTAGTAGATTGTATAGTT1905761.4563060798119554No Hit
TGAGGTAGTAGTTTGTACAGTT1533951.1721836491098296No Hit
TGAGGTAGTAGTTTGTGCTGTT1458291.114367282936454No Hit
TTCAAGTAATCCAGGATAGGCT1360521.0396553331509537No Hit
ATCACATTGCCAGGGATTTCC1304360.9967400922799944No Hit
TTCAAGTAATTCAGGATAGGTT1218270.9309535344705057No Hit
ACCTCAGAAGGTCTCACTTTT1185050.9055681302373634No Hit
TAGCAGCACGTAAATATTGGC1002270.7658949157360468No Hit
ATCACATTGCCAGGGATTACC982940.7511236976798565No Hit
AACCCGTAGATCCGATCTTGTG971730.7425574610316469No Hit
TAGCTTATCAGACTGATGTTGA876630.6698858191721698No Hit
TCCCTACACGACGCTCTCCCTACACG811460.6200854942512223No Hit
TAGCTTATCAGACTGATGTTG809640.6186947225563301No Hit
AACCCGTAGATCCGATCTTGT725670.5545281845233092No Hit
CACAGATGATGAACTTATTG698170.5335137770455425No Hit
TAGCAGCACGTAAATATTGGCGA555450.42445282303729254No Hit
TTCACAGTGGCTAAGTTCCG538930.4118288953451941No Hit
ATCACATTGCCAGGGATTTCCA520180.3975008902467168No Hit
ATCACATTGCCAGGGATTACCACT514700.39331329195660175No Hit
TGAGGTAGTAGGTTGTATAGTT512540.3916627057692572No Hit
TCCCTACACGACGCTCTCCCTAC496020.3790387780771588No Hit
TGGCTCAGTTCAGCAGGAACAGT490430.37476712215713276No Hit
AAGCTGCCAGTTGAAGAACTGT465200.35548735849662166No Hit
ACAGATGATGAACTTATTG444650.33978386490869045No Hit
TGGCTCAGTTCAGCAGGAACA443830.33915725348571707No Hit
CAGTGCAATGTTAAAAGGGCATT418940.32013730431765836No Hit
AACCCGTAGATCCGATCTTGTGT393270.300521310137503No Hit
TGGCTCAGTTCAGCAGGAAC362680.2771456474195072No Hit
GGTATCGTAAACCGAAGATC328580.2510877821470764No Hit
AGGGGTAAAGCACTGTTACGTAT302190.2309215925711395No Hit
TAGCACCATCTGAAATCGGTT299300.2287131693852942No Hit
TCCCTAC290420.22192742617065533No Hit
AACCCGTAGATCCGATCTTGTGA288520.22047552165400963No Hit
GTTTCCGTAGTGTAGTGGTTATC281820.21535564783215375No Hit
TGAGGTAGTAAGTTGTATTGTT273210.20877622789093292No Hit
ATCACATTGCCAGGGATTTC264530.2021433167306778No Hit
AAGCTGCCAGTTGAAGAACTG258660.19765769593451452No Hit
CAAAGTGCTTACAGTGCAGGTAGT257730.19694702688163004No Hit
GCCCGGATAGCTCAGTCGGTAGAGCA241880.1848350865717172No Hit
AAAGGTTCGTTTGTAAAAAA239980.1833831820550715No Hit
TCGTACCGTGAGTAATAATGCG237560.18153391419702805No Hit
AGGCAAGATGCTGGCATAGCT234940.1795318142846008No Hit
AAAGGTTCGTTTGTAAAAA227130.17356372256091504No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT220740.16868073842335396No Hit
TGGCTCAGTTCAGCAGGA219170.16748100679644146No Hit
TTCACAGTGGCTAAGTTCTGC218210.16674741293539944No Hit
TTTGGCACTAGCACATTTTTGCT212020.1620172608522221No Hit
TGTAAACATCCCCGACTGGAAGCT211160.16136008301837196No Hit
AAAGGTTCGTTTGTAAAAAAA210080.16053478992469966No Hit
CTGACCTATGAATTGACAGCC203530.15552954014363155No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC201350.15386367075084859No Hit
GAAAATGTTTAG198130.1514030746752701No Hit
TAAAGGTTCGTTTGTAAAAA198110.15138779146983172No Hit
AGCTACATTGTCTGCTGGGTTT197460.1508910872930845No Hit
AACCTGGCGGAAACACCA192360.14699386990629865No Hit
TAGCAGCACATCATGGTTTACA191980.14670348900296953No Hit
GCCCGGATAGCTCAGTCGGTAGAGC190290.14541205814342675No Hit
GGTGTCGTAAACCGAAGATC187400.14320363495758148No Hit
TGTAAACATCCTCGACTGGAAGCT186030.14215673538505272No Hit
TTCAAGTAATCCAGGATAGGCTT179590.1372355432338957No Hit
AGGCAAGATGCTGGCATAGCTG178950.1367464806598677No Hit
TAGCAGCACGTAAATATTGGCGTT178840.13666242302995663No Hit
TACCAGCACGTAAATATTGGCG174830.13359814033956227No Hit
TAAAGGTTCGTTTGTAAAAAA166740.12741608373973923No Hit
TAGCTTATCAGACTGATGTTGACA165200.1262392769209843No Hit
AGCAGCATTGTACAGGGCTATGT165090.12615521929107323No Hit
AGGCAAGATGCTGGCATAGC164230.12549804145722307No Hit
TGTAACAGCAACTCCATGTGGA160870.12293046294357593No Hit
AGCTACATTGTCTGCTGGGTTTC160760.12284640531366486No Hit
TAGCTTATCAGACTGATGTTGACT160540.12267829005384273No Hit
AAAGGTTCGTTTGTAAAAAAAA158670.12124931034535459No Hit
TTTGGCAATGGTAGAACTCACACT158210.12089779662027196No Hit
AATGACACGATCACTCCCGTTGAGT157870.12063798212781957No Hit
TGTAAACATCCTTGACTGGAAGCT157710.12051571648431256No Hit
ATCACATTGCCAGGGATTTCCAAT154460.11803219560057648No Hit
AGCTCGGTCTGAGGCCCCTCAGT154000.11768068187549385No Hit
TGGCTCAGTTCAGCAGGAACAG152370.11643510063226621No Hit
CAAAGTGCTTACAGTGCAGGTAG152010.11616000293437545No Hit
TGGCAGCACGTAAATATTGGCG151690.11591547164736143No Hit
GGCTTGGTCTAGGGGTATGATT147750.11290468017600139No Hit
GGCTGTAGGAATACTTTT145880.11147570046751325No Hit
AGTAAGGTCAGCTAAATAAGCTA145410.11111654513971142No Hit
TTCAAGTAATCCAGGATAGGC143210.1094353925414901No Hit
TAAAGGTTCGTTTGTAAAAAAA143040.10930548529526389No Hit
ACCTCAGAAGGTCTCACTTT142640.10899982118649637No Hit
GAATCCCGAGTGTTGTTCTC141600.10820509450370083No Hit
AATCCCGAGTGTTGTTCTC135690.10368890729666076No Hit
AATTGCACGGTATCCATCTGTA134240.10258087490237852No Hit
TGTAACAGCAACTCCATGTGG133670.10214530354738481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)