FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002441087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002441087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11844538
Sequences flagged as poor quality0
Sequence length0-34
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGCACGTAAATATTGGCG181718115.341932289803115No Hit
TAGCAGCACGTAAATATTGGCGT3411372.880120778032879No Hit
GGTTTCGTAAACCGAAGATC2310101.9503504484514298No Hit
TTCAAGTAATCCAGGATAGGCT2251941.9012476468056416No Hit
TTCAAGTAATTCAGGATAGGTT1976281.6685159015910962No Hit
TAGCTTATCAGACTGATGTTGAC1637791.382738609137815No Hit
TGAGATGAAGCACTGTAGCTC1555671.3134070742142918No Hit
ATCGTGCATCCCTTTAGAGTGT1369401.1561447141289936No Hit
ACAGTAGTCTGCACATTGGTT1331591.1242228274332018No Hit
ACCTCAGAAGGTCTCACTTTT1240971.0477149889679107No Hit
AAAGTGCTTCCTTTTAGAGGGT1166490.984833684521929No Hit
ATCGTGCATCCTTTTAGAGTGT993480.838766357961788No Hit
AACCGGGCGGAAACACCA935760.7900350355581618No Hit
CAAGTGCTTCCTTTTAGAGGGT899640.7595399668606746No Hit
AGGGGTAAAGCACTGTTACGTAT879960.7429247134839705No Hit
CAGTGCAATGTTAAAAGGGCATT853750.7207963704451791No Hit
TAGCAGCACGTAAATATTGGC849320.7170562498934108No Hit
CACAGATGATGAACTTATTG761790.6431572088333036No Hit
TCCCTACACGACGCTCTCCCTACACG761390.6428195004313381No Hit
CCCAGTGTTCAGACTACCTGTTC544310.45954515068464474No Hit
CCCAGTGTTTAGACTATCTGTTC499380.42161205443386646No Hit
ATCACATTGCCAGGGATTTCC471580.3981413204972621No Hit
GAAAGCGCTTCTCTTTAGAGGA431460.3642691677801194No Hit
AACCCGTAGATCCGATCTTGTG430330.3633151415445668No Hit
TCCCTACACGACGCTCTCCCTAC421270.3556660462400475No Hit
TACAGTAGTCTGCACATTGGTT420960.35540432222852425No Hit
ATCACATTGCCAGGGATTACC400830.3384091468996089No Hit
TAGCAGCACGTAAATATTGGCGA396720.3349391930694131No Hit
GAAGCGGGTGCTCTTATTTT380830.3215237268013324No Hit
AACCCGTAGATCCGAACTTGTG372950.3148708712826115No Hit
CAAAGCGCTTCTCTTTAGAGTG363780.3071289061675517No Hit
GGTATCGTAAACCGAAGATC362700.30621709348224474No Hit
TGAGATGAAGCACTGTAGCTCT353140.29814586267526855No Hit
AACCCGTAGATCCGATCTTGT338620.28588704768391976No Hit
AATCGTACAGGGTCATCCACTT327890.2768280198011944No Hit
TGAGGTAGTAGGTTGTATAGTT320550.270631070625127No Hit
GGCTGTAGGAATACTTTT318630.2690100702956924No Hit
TGAGGTAGTAGATTGTATAGTT318190.26863859105353033No Hit
TTCTCGAGGAAAGAAGCACTTTC313310.2645185485495508No Hit
ACAGATGATGAACTTATTG300470.25367810884645736No Hit
ACTGGACTTGGAGTCAGAAGGC297020.25076537387950465No Hit
TAGGGGTAAAGCACTGTTACGTAT289400.2443320288220613No Hit
AAAGTGCATCCTTTTAGAGTGT287470.24270258578257758No Hit
CAGTGCAATGTTAAAAGGGCATA278640.2352476728091885No Hit
AAAGTGCATCCTTTTAGAGGTT246190.20785107869973485No Hit
TGAGATGAAGCACTGTAGCT244790.20666909929285548No Hit
ATCACATTGCCAGGGATTTCCA234200.19772826935081808No Hit
AGCTACATTGTCTGCTGGGTTTC228280.19273018500172823No Hit
AACCCGTAGATCCGAACTTGT228110.19258665893089288No Hit
TTCAAGTAATCCAGGATAGGC228090.1925697735107946No Hit
AGCAGCATTGTACAGGGCTATGA226930.19159041914509456No Hit
TAGGGAGGTTATGATTAACTTTT223990.1891082623906479No Hit
TAGCTTATCAGACTGATGTTGA221170.18672741815679092No Hit
CAGTGGTTTTACCCTATGGTAG218180.18420304785209857No Hit
TTCTCGAGGAAAGAAGCACTTTCT216850.18308016741556318No Hit
TTCAAGTAATCCAGGATAGGCTT210150.17742355168264057No Hit
CAAAGTGCTTACAGTGCAGGTAGT208150.1757350096728129No Hit
AAAGTGCTTCCTTTTTGAGGGT206320.1741899937338206No Hit
AAGGAGCTCACAGTCTATTGAG203900.17214685790192913No Hit
GGTGTCGTAAACCGAAGATC200570.16933543545556612No Hit
ACCATCGACCGTTGATTGTACC199350.16830542482957123No Hit
TAGGTAGTTTCATGTTGTTGGG198290.16741049756436258No Hit
ACAGTAGTCTGCACATTGGTTA197620.1668448359910703No Hit
GAAAGCGCTTCCTTTTAGAGGA196340.16576416910478062No Hit
TTCACAGTGGCTAAGTTCTGC192950.16290209039812276No Hit
TAGGATGGGGTGTGATAGGTGGC189040.15960099076890968No Hit
AGGCAGTGTAGTTAGCTGATTGC183130.15461134912986896No Hit
CCCAGTGTTCAGACTACCTGTT181170.15295657796023787No Hit
TCCCTAC180270.15219673405581544No Hit
TAGCTTATCAGACTGATGTTG176850.14930932721901014No Hit
GTTTCCGTAGTGTAGTGGTTATC171780.14502887322409705No Hit
AAGCTGCCAGTTGAAGAACTGT159340.13452614192296905No Hit
TGGCTCAGTTCAGCAGGAACA158400.13373252717835005No Hit
TACCCTGTAGAACCGAATTTGT155840.13157119340577067No Hit
TTGTACATGGTAGGCTTTCATT155110.13095487557218355No Hit
TCGTGCATCCCTTTAGAGTGT153760.12981510971554988No Hit
GAAGCGGGTGCTCTTATTT152620.12885264076994815No Hit
TTAATATCGGACAACCATTGT150530.12708811436967826No Hit
GTAGTCGTGGCCGAGTGGTTAAGGC148890.12570350992161958No Hit
TGTAAACATCCTCGACTGGAAGCT147880.1248507962066566No Hit
CAAAGTGCTTACAGTGCAGGTAG145660.1229765145757479No Hit
AACATTCATTGCTGTCGGTGGGTT144530.1220224883401953No Hit
GAATCCCGAGTGTTGTTCTC141090.11911819608329173No Hit
AATCCCGAGTGTTGTTCTC138160.11664448203889421No Hit
CAGTGCAATGATGAAAGGGCATT138160.11664448203889421No Hit
CAAAGTGCTGTTCGTGCAGGTAG137990.11650095596805886No Hit
TAGCAGCACATCATGGTTTACA137560.11613791943594592No Hit
TAGGGAGGTTATGACTAACTTTT131060.11065015790400605No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT130790.1104222047326793No Hit
TAAAGTGCTGACAGTGCAGATA130700.11034622034223707No Hit
CAGTAGTCTGCACATTGGTT129330.10918956906550512No Hit
GAAAGCGCTTCCCTTTGCTGGA127270.10745037079538265No Hit
GAAAGCGCTTCTCTTTAGAGG127000.10722241762405592No Hit
AGGCGATCACGTAGATTTT125690.1061164226076188No Hit
ACCTCAGAAGGTCTCACTTT125090.10560986000467051No Hit
AAAGGTTCGTTTGTAAAAAA123350.10414082845612044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)